Evidence map›Paper›PMID 26977352›Full record

ArticleBiomedical optics express2016

Frapid: achieving full automation of FRAP for chemical probe validation.

Clarence Yapp, Catherine Rogers, Pavel Savitsky, Martin Philpott, Susanne Müller

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Clarence YappTarget Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK; Botnar Research Centre, NDORMS, University of Oxford, Oxford, OX3 7LD, UK.
Catherine RogersTarget Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK.
Pavel SavitskyStructural Genomics Consortium, ORCRB, University of Oxford, Oxford, OX3 7DQ, UK.
Martin PhilpottBotnar Research Centre, NDORMS, University of Oxford, Oxford, OX3 7LD, UK.
Susanne MüllerTarget Discovery Institute, NDMRB, University of Oxford, Oxford, OX3 7FZ, UK.
University of Oxford · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence Recovery After Photobleaching (FRAP) is an established method for validating chemical probes against the chromatin reading bromodomains, but so far requires constant human supervision. Here, we present Frapid, an automated open source code implementation of FRAP that fully handles cell identification through fuzzy logic analysis, drug dispensing with a custom-built fluid handler, image acquisition & analysis, and reporting. We successfully tested Frapid on 3 bromodomains as well as on spindlin1 (SPIN1), a methyl lysine binder, for the first time.

Indexed as

(100.3008) Image recognition, algorithms and filters(150.0150) Machine vision(150.5758) Robotic and machine control(170.1530) Cell analysis(170.1790) Confocal microscopy(170.2520) Fluorescence microscopy

Identifiers

PMID26977352
PMCPMC4771461
OpenAlexW2237069126

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.