ArticleBiomedical optics express2016
Fast interferometric second harmonic generation microscopy.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Second harmonic generation microscopy: a powerful tool for bio-imaging.Biophysical reviews · 2023Review
- Nonlinear optical imaging by detection with optical parametric amplification (invited paper).Journal of innovative optical health sciences · 2023Article
- Polarity-dependent nonlinear optics of nanowires under electric field.Nature communications · 2021Article
- Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae.Scientific reports · 2021Article
- Complex Susceptibilities and Chiroptical Effects of Collagen Measured with Polarimetric Second-Harmonic Generation Microscopy.Scientific reports · 2019Article
- Elimination of imaging artifacts in second harmonic generation microscopy using interferometry.Biomedical optics express · 2019Article
- Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy.Scientific reports · 2017Article
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Authors and funding
7 authors.
Funding
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Abstract
We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.
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Registered trials
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