ArticleBiomedical optics express2016
Wide field-of-view fluorescence image deconvolution with aberration-estimation from Fourier ptychography.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Review
- Peripheral Blood Leukocyte Detection Based on an Improved Detection Transformer Algorithm.Sensors (Basel, Switzerland) · 2023Article
- Three-dimensional bi-functional refractive index and fluorescence microscopy (BRIEF).Biomedical optics express · 2022Article
- Review of bio-optical imaging systems with a high space-bandwidth product.Advanced photonics · 2021Article
- Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes).Scientific reports · 2019Article
- Article
- Wide-field Fourier ptychographic microscopy using laser illumination source.Biomedical optics express · 2016Article
- Motion-corrected Fourier ptychography.Biomedical optics express · 2016Article
- Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy.Biomedical optics express · 2016Article
- Fourier ptychographic reconstruction using Poisson maximum likelihood and truncated Wirtinger gradient.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
This paper presents a method to simultaneously acquire an aberration-corrected, wide field-of-view fluorescence image and a high-resolution coherent bright-field image using a computational microscopy method. First, the procedure applies Fourier ptychographic microscopy (FPM) to retrieve the amplitude and phase of a sample, at a resolution that significantly exceeds the cutoff spatial frequency of the microscope objective lens. At the same time, redundancy within the set of acquired FPM bright-field images offers a means to estimate microscope aberrations. Second, the procedure acquires an aberrated fluorescence image, and computationally improves its resolution through deconvolution with the estimated aberration map. An experimental demonstration successfully improves the bright-field resolution of fixed, stained and fluorescently tagged HeLa cells by a factor of 4.9, and reduces the error caused by aberrations in a fluorescence image by up to 31%, over a field of view of 6.2 mm by 9.3 mm. For optimal deconvolution, we show the fluorescence image needs to have a signal-to-noise ratio of at least ~18.
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