Evidence map›Paper›PMID 26977337›Full record

ArticleBiomedical optics express2016

Miniature in vivo MEMS-based line-scanned dual-axis confocal microscope for point-of-care pathology.

C Yin, A K Glaser, S Y Leigh, Y Chen, L Wei, P C S Pillai, M C Rosenberg, S Abeytunge, G Peterson, C Glazowski and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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  20. Dual-axis confocal microscopy for point-of-care pathology.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

C YinUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
A K GlaserUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
S Y LeighUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
Y ChenUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
L WeiUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
P C S PillaiUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
M C RosenbergUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.
S AbeytungeMemorial Sloan-Kettering Cancer Center, Dermatology Services, Department of Medicine, New York, NY 10010, USA.
G PetersonMemorial Sloan-Kettering Cancer Center, Dermatology Services, Department of Medicine, New York, NY 10010, USA.
C GlazowskiMemorial Sloan-Kettering Cancer Center, Dermatology Services, Department of Medicine, New York, NY 10010, USA.
N SanaiBarrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ 85013 USA.
M J MandellaStanford University School of Medicine, Department of Pediatrics, Stanford, CA 94305, USA.
M RajadhyakshaMemorial Sloan-Kettering Cancer Center, Dermatology Services, Department of Medicine, New York, NY 10010, USA.
J T C LiuUniversity of Washington, Department of Mechanical Engineering, Seattle, WA 98195, USA.

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Hongbiao Carl Lekaye · 1985 to 2026
$347.4M
Line-scanned MEMS-based dual-axis confocal microscopy to detect oral lesions in vR01DE023497 · NIDCR · UNIVERSITY OF WASHINGTON · PI LIU, JONATHAN T.C. · 2012 to 2014
$2.4M
Intraoperative confocal microscopy for quantitative delineation of low-grade glioR01CA175391 · NCI · UNIVERSITY OF WASHINGTON · PI LIU, JONATHAN T.C., SANAI, NADER · 2014 to 2018
$1.7M
NCI NIH HHS P30 CA008748NCI NIH HHS R01 CA175391NIDCR NIH HHS R01 DE023497
6 · The paper itself

Abstract

There is a need for miniature optical-sectioning microscopes to enable in vivo interrogation of tissues as a real-time and noninvasive alternative to gold-standard histopathology. Such devices could have a transformative impact for the early detection of cancer as well as for guiding tumor-resection procedures. Miniature confocal microscopes have been developed by various researchers and corporations to enable optical sectioning of highly scattering tissues, all of which have necessitated various trade-offs in size, speed, depth selectivity, field of view, resolution, image contrast, and sensitivity. In this study, a miniature line-scanned (LS) dual-axis confocal (DAC) microscope, with a 12-mm diameter distal tip, has been developed for clinical point-of-care pathology. The dual-axis architecture has demonstrated an advantage over the conventional single-axis confocal configuration for reducing background noise from out-of-focus and multiply scattered light. The use of line scanning enables fast frame rates (16 frames/sec is demonstrated here, but faster rates are possible), which mitigates motion artifacts of a hand-held device during clinical use. We have developed a method to actively align the illumination and collection beams in a DAC microscope through the use of a pair of rotatable alignment mirrors. Incorporation of a custom objective lens, with a small form factor for in vivo clinical use, enables our device to achieve an optical-sectioning thickness and lateral resolution of 2.0 and 1.1 microns respectively. Validation measurements with reflective targets, as well as in vivo and ex vivo images of tissues, demonstrate the clinical potential of this high-speed optical-sectioning microscopy device.

Indexed as

(170.1790) Confocal microscopy(170.2520) Fluorescence microscopy(170.3880) Medical and biological imaging(170.5810) Scanning microscopy(230.4685) Optical microelectromechanical devices

Identifiers

PMID26977337
PMCPMC4771446

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.