ArticleStem cells international2016
Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use.
Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Continuous collection of human mesenchymal-stromal-cell-derived extracellular vesicles from a stirred tank reactor operated under xenogeneic-free conditions for therapeutic applications.Stem cell research & therapy · 2025Article
- Identification of critical process parameters and quality attributes for bioreactor-based expansion of human MSCs.Frontiers in bioengineering and biotechnology · 2025Review
- Bioengineered mesenchymal stem cell-derived exosomes: emerging strategies for diabetic wound healing.Burns & trauma · 2024Review
- Scalable manufacturing of gene-modified human mesenchymal stromal cells with microcarriers in spinner flasks.Applied microbiology and biotechnology · 2023Article
- Understanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing.Biotechnology letters · 2023Article
- Article
- Mesenchymal stem cell-derived extracellular vesicles for cell-free therapy of ocular diseases.Extracellular vesicles and circulating nucleic acids · 2022Review
- Article
- Facile bead-to-bead cell-transfer method for serial subculture and large-scale expansion of human mesenchymal stem cells in bioreactors.Stem cells translational medicine · 2021Article
- Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers.Materials today. Bio · 2021Article
- Strategies to Enhance Extracellular Vesicle Production.Tissue engineering and regenerative medicine · 2021Review
- Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state.Scientific reports · 2021Article
- Microcarrier Screening and Evaluation for Dynamic Expansion of Human Periosteum-Derived Progenitor Cells in a Xenogeneic Free Medium.Frontiers in bioengineering and biotechnology · 2021Article
- Influence of Microenvironment on Mesenchymal Stem Cell Therapeutic Potency: From Planar Culture to Microcarriers.Frontiers in bioengineering and biotechnology · 2020Review
- Biological Considerations in Scaling Up Therapeutic Cell Manufacturing.Frontiers in pharmacology · 2020Review
- Human Platelet Lysate Improves Bone Forming Potential of Human Progenitor Cells Expanded in Microcarrier-Based Dynamic Culture.Stem cells translational medicine · 2019Article
- Generation of mesenchymal stem-like cells for producing extracellular vesicles.World journal of stem cells · 2019Review
- Improved expansion of equine cord blood derived mesenchymal stromal cells by using microcarriers in stirred suspension bioreactors.Journal of biological engineering · 2019Article
- Manufacturing human mesenchymal stem cells at clinical scale: process and regulatory challenges.Applied microbiology and biotechnology · 2018Review
- Engineering mesenchymal stem cells to improve their exosome efficacy and yield for cell-free therapy.Journal of extracellular vesicles · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.