ArticleImmunology letters2016
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum.
Article in Immunology letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- TheInfection and immunity · 2026Article
- Complement Resistance Assays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Discerning dangerous gain of function: most gain of function (GoF) research does not involve infectious microbes.Frontiers in bioengineering and biotechnology · 2026Review
- Exposure to live saprophyticeLife · 2024Article
- Exposure to live saprophyticbioRxiv : the preprint server for biology · 2024Article
- The human factor H protein family - an update.Frontiers in immunology · 2024Review
- Evaluation ofFrontiers in microbiology · 2023Article
- The FUR-like regulators PerRA and PerRB integrate a complex regulatory network that promotes mammalian host-adaptation and virulence of Leptospira interrogans.PLoS pathogens · 2021Article
- Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis.Pathogens (Basel, Switzerland) · 2021Article
- Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C.Scientific reports · 2021Article
- A Review on Host-Frontiers in cellular and infection microbiology · 2021Review
- Hijacking Factor H for Complement Immune Evasion.Frontiers in immunology · 2021Review
- Deciphering the Role ofFrontiers in immunology · 2021Article
- Evaluation of LipL32 and LigA/LigB Knockdown Mutants inFrontiers in microbiology · 2021Article
- Heterologous Expression of the Pathogen-Specific LIC11711 Gene in the SaprophytePathogens (Basel, Switzerland) · 2020Article
- Leptospiral Immunoglobulin-Like Domain Proteins: Roles in Virulence and Immunity.Frontiers in immunology · 2020Review
- Role of Murine Complement Component C5 in AcuteFrontiers in cellular and infection microbiology · 2018Article
- Virulence of the zoonotic agent of leptospirosis: still terra incognita?Nature reviews. Microbiology · 2017Review
- Immunoprotective properties of recombinant LigA and LigB in a hamster model of acute leptospirosis.PloS one · 2017Article
- Complement Evasion by PathogenicFrontiers in immunology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Leptospiral immunoglobulin-like (Lig) proteins are surface exposed molecules present in pathogenic but not in saprophytic Leptospira species. We have previously shown that Lig proteins interact with the soluble complement regulators Factor H (FH), FH like-1 (FHL-1), FH related-1 (FHR-1) and C4b Binding Protein (C4BP). In this study, we used the saprophyte L. biflexa serovar Patoc as a surrogate host to address the specific role of LigA and LigB proteins in leptospiral complement evasion. L. biflexa expressing LigA or LigB was able to acquire FH and C4BP. Bound complement regulators retained their cofactor activities of FI in the proteolytic cleavage of C3b and C4b. Moreover, heterologous expression of ligA and ligB genes in the saprophyte L. biflexa enhanced bacterial survival in human serum. Complement deposition on lig-transformed L. biflexa was assessed by flow cytometry analysis. With regard to MAC deposition, L. biflexa expressing LigA or LigB presented an intermediate profile: MAC deposition levels were greater than those found in the pathogenic L. interrogans, but lower than those observed for L. biflexa wildtype. In conclusion, Lig proteins contribute to in vitro control of complement activation on the leptospiral surface, promoting an increased bacterial survival in human serum.
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