ArticleScientific reports2016
Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 132 citations in OpenAlex.
- Recent advances in surface enhanced Raman spectroscopy for bacterial pathogen identifications.Journal of advanced research · 2023Pooled it
- Detection and identification of pathogens using agents targeting the bacterial cell wall.Folia microbiologica · 2026Review
- Aptamer-functionalized magnetic beads combined with hybridization chain reaction for detection of Bacillus cereus.NPJ science of food · 2026Article
- Advancements in Microbial Applications for Sustainable Food Production.Foods (Basel, Switzerland) · 2025Review
- Pentameric nanobodies serve as a capture agent and RANbodies function as immunoprobes for the sensitive detection of Salmonella typhimurium in immunoassays.Analytical and bioanalytical chemistry · 2025Article
- An indirect competitive ELISA for determination of guanidine acetic acid in animal feed.Scientific reports · 2025Article
- Development and Characterization of a Polyvalent Polyclonal Antibody as a Common Capture Antibody for the Detection of Enterotoxigenic Escherichia coli in a Sandwich ELISA.Current microbiology · 2025Article
- Highly sensitive and rapid detection of Vibrio parahaemolyticus using a dual-recognition platform based on functionalized quantum dots and aptamer.Mikrochimica acta · 2024Article
- Polarization modulated spectroscopic ellipsometry-based surface plasmon resonance biosensor for E. coli K12 detection.Scientific reports · 2024Article
- Apple cider vinegar exhibits promising antibiofilm activity against multidrug-resistantOpen veterinary journal · 2024Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Recent Progress and Challenges on the Microfluidic Assay of Pathogenic Bacteria Using Biosensor Technology.Biomimetics (Basel, Switzerland) · 2022Review
- Functionalization of an extended-gate field-effect transistor (EGFET) for bacteria detection.Scientific reports · 2022Article
- Development of a Double-Antibody Sandwich ELISA for Rapid Detection of the MCP Antigen Concentration in Inactivated ISKNV Vaccines.Vaccines · 2021Article
- A Double-Antibody Sandwich ELISA for Sensitive and Specific Detection of Swine Fibrinogen-Like Protein 1.Frontiers in immunology · 2021Article
- Flos lonicerae flavonoids attenuate experimental ulcerative colitis in rats via suppression of NF-κB signaling pathway.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.Sensors (Basel, Switzerland) · 2020Review
- Impact of vaccination during pregnancy and staphylococci concentration on the presence of Bacillus cereus in raw human milk.Journal of perinatology : official journal of the California Perinatal Association · 2020Article
- Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis.Scientific reports · 2020Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. In this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) that was developed for rapid detection of B. cereus in food to minimize the risk of contamination. The polyclonal antibody (pAb) and monoclonal antibodies (mAbs) specific to B. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid/saturated ammonium sulfate precipitation method and protein A-sepharose columns. IgG-isotype mAbs were specially developed to undergo a novel peripheral multiple sites immunization for rapid gain of hybridomas and a subtractive screen was used to eliminate cross reactivity with closely related species such as Bacillus thuringiensis, B. subtilis, B. licheniformis and B. perfringens. The linear detection range of the method was approximately 1 × 10(4)-2.8 × 10(6) cells/mL with a detection limit (LOD) of 0.9 × 10(3) cells/mL. The assay was able to detect B. cereus when the samples were prepared in meat with various pathogens. The newly developed analytical method provides a rapid method to sensitively detect B. cereus in food specimens.
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