ArticleProceedings of the National Academy of Sciences of the United States of America2016
New inducible genetic method reveals critical roles of GABA in the control of feeding and metabolism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 64 citations in OpenAlex.
- Biosensor cell array reveals temporal GABA secretion dynamics from pancreatic islets.Molecular metabolism · 2026Article
- Dual interference with host neuropeptide signaling allows parasitoid wasp to hijack host sugar metabolism.The EMBO journal · 2026Article
- Beta cell secreted GABA sets appropriate insulin secretion by modulating islet calcium oscillations.Molecular metabolism · 2025Article
- GABA produced by multiple bone marrow cell types regulates hematopoietic stem and progenitor cells.Stem cell reports · 2025Article
- Transcriptional profiling of the hippocampus and hypothalamus reveals a potential role of thyroid hormone in gamma-aminobutyric acid (GABA) synthesis in dairy cows fed a high-concentrate diet.BMC veterinary research · 2025Article
- Defining spatial nonuniformities of all ipRGC types using an improved Opn4Cell reports methods · 2024Article
- A newbioRxiv : the preprint server for biology · 2024Article
- Synergistic effects of peripheral GABA and GABA-transaminase inhibitory drugs on food intake control and weight loss in high-fat diet-induced obese mice.Frontiers in pharmacology · 2024Article
- Novel therapeutic strategy for obesity through the gut microbiota-brain axis: A review article.Caspian journal of internal medicine · 2024Review
- Increased body weight in mice with fragile X messenger ribonucleoprotein 1 (Fmr1) gene mutation is associated with hypothalamic dysfunction.Scientific reports · 2023Article
- Article
- Identification of a GABAergic neural circuit governing leptin signaling deficiency-induced obesity.eLife · 2023Article
- Transgenic mice encoding modern imaging probes: Properties and applications.Cell reports · 2022Review
- Role of the gut-brain axis in energy and glucose metabolism.Experimental & molecular medicine · 2022Review
- The critical role of gut microbiota in obesity.Frontiers in endocrinology · 2022Review
- Reciprocal control of obesity and anxiety-depressive disorder via a GABA and serotonin neural circuit.Molecular psychiatry · 2021Article
- Deciphering an AgRP-serotoninergic neural circuit in distinct control of energy metabolism from feeding.Nature communications · 2021Article
- A hindbrain dopaminergic neural circuit prevents weight gain by reinforcing food satiation.Science advances · 2021Article
- The Role of the Gut Microbiota in the Gut-Brain Axis in Obesity: Mechanisms and Future Implications.International journal of molecular sciences · 2021Review
- Energy state alters regulation of proopiomelanocortin neurons by glutamatergic ventromedial hypothalamus neurons: pre- and postsynaptic mechanisms.Journal of neurophysiology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Currently available inducible Cre/loxP systems, despite their considerable utility in gene manipulation, have pitfalls in certain scenarios, such as unsatisfactory recombination rates and deleterious effects on physiology and behavior. To overcome these limitations, we designed a new, inducible gene-targeting system by introducing an in-frame nonsense mutation into the coding sequence of Cre recombinase (nsCre). Mutant mRNAs transcribed from nsCre transgene can be efficiently translated into full-length, functional Cre recombinase in the presence of nonsense suppressors such as aminoglycosides. In a proof-of-concept model, GABA signaling from hypothalamic neurons expressing agouti-related peptide (AgRP) was genetically inactivated within 4 d after treatment with a synthetic aminoglycoside. Disruption of GABA synthesis in AgRP neurons in young adult mice led to a dramatic loss of body weight due to reduced food intake and elevated energy expenditure; they also manifested glucose intolerance. In contrast, older mice with genetic inactivation of GABA signaling by AgRP neurons had only transient reduction of feeding and body weight; their energy expenditure and glucose tolerance were unaffected. These results indicate that GABAergic signaling from AgRP neurons plays a key role in the control of feeding and metabolism through an age-dependent mechanism. This new genetic technique will augment current tools used to elucidate mechanisms underlying many physiological and neurological processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.