ArticleDevelopmental and comparative immunology2016
Characterization and regulation of expression of an antifungal peptide from hemolymph of an insect, Manduca sexta.
Article in Developmental and comparative immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Insect Antimicrobial Peptides as Guardians of Immunity and Beyond: A Review.International journal of molecular sciences · 2024Review
- Exploring the antimicrobial peptidome of nematodes through phylum-spanning in silico analyses highlights novel opportunities for pathogen control.PLoS neglected tropical diseases · 2023Article
- Blapstin, a Diapause-Specific Peptide-Like Peptide from the Chinese Medicinal BeetleMicrobiology spectrum · 2023Article
- Article
- Topical Fungal Infection Induces Shifts in the Gut Microbiota Structure of Brown Planthopper,Insects · 2022Article
- Inhibition of Zoonotic Pathogens Naturally Found in Pig Manure by Black Soldier Fly Larvae and Their Intestine Bacteria.Insects · 2022Article
- His-Ala-Phe-Lys peptide fromFrontiers in microbiology · 2022Article
- Activation of the Host Immune Response inInsects · 2021Article
- Article
- Identification and recombinant expression of an antimicrobial peptide (cecropin B-like) from soybean pestThe journal of venomous animals and toxins including tropical diseases · 2021Article
- Integrative multiomics analysis of Premolis semirufa caterpillar venom in the search for molecules leading to a joint disease.Scientific reports · 2021Article
- Tobacco Hornworm (Virulence · 2020Article
- Mosquito-fungus interactions and antifungal immunity.Insect biochemistry and molecular biology · 2019Review
- Silk properties and overwinter survival in gregarious butterfly larvae.Ecology and evolution · 2018Article
- Recent Advances in the Use ofJournal of fungi (Basel, Switzerland) · 2018Review
- An analysis of 67 RNA-seq datasets from various tissues at different stages of a model insect, Manduca sexta.BMC genomics · 2017Article
- Transcription Factor Forkhead Regulates Expression of Antimicrobial Peptides in the Tobacco Hornworm, Manduca sexta.Scientific reports · 2017Article
- Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Insects secrete antimicrobial peptides as part of the innate immune response. Most antimicrobial peptides from insects have antibacterial but not antifungal activity. We have characterized an antifungal peptide, diapausin-1 from hemolymph of a lepidopteran insect, Manduca sexta (tobacco hornworm). Diapausin-1 was isolated by size exclusion chromatography from hemolymph plasma of larvae that were previously injected with a yeast, Saccharomyces cerevisiae. Fractions containing activity against S. cerevisiae were analyzed by SDS-PAGE and MALDI-TOF MS/MS and found to contain a 45-residue peptide that was encoded by sequences identified in M. sexta transcriptome and genome databases. A cDNA for diapausin-1 was cloned from cDNA prepared from fat body RNA. Diapausin-1 is a member of the diapausin family of peptides, which includes members known to have antifungal activity. The M. sexta genome contains 14 genes with high similarity to diapausin-1, each with 6 conserved Cys residues. Diapausin-1 was produced as a recombinant protein in Escherichia coli. Purified recombinant diapausin-1 was active against S. cerevisiae, with IC50 of 12 μM, but had no detectable activity against bacteria. Spores of some plant fungal pathogens treated with diapausin-1 had curled germination tubes or reduced and branched hyphal growth. Diapausin-1 mRNA level in fat body strongly increased after larvae were injected with yeast or with Micrococcus luteus. In addition, diapausin-1 mRNA levels increased in midgut and fat body at the wandering larval stage prior to pupation, suggesting developmental regulation of the gene. Our results indicate that synthesis of diapausin-1 is part of an antifungal innate immune response to infection in M. sexta.
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