Evidence map›Paper›PMID 26975931›Full record

ArticleScientific reports2016

Development of a method for detection and quantification of B. brongniartii and B. bassiana in soil.

L Canfora, E Malusà, C Tkaczuk, M Tartanus, B H Łabanowska, F Pinzari

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
5.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 34 citations in OpenAlex.

  1. Endophytic entomopathogenic fungi: The next frontier in mycological biocontrol.World journal of microbiology & biotechnology · 2026
    Review
  2. Structured Framework and Genome Analysis ofJournal of fungi (Basel, Switzerland) · 2022
    Article
  3. The impact ofFrontiers in microbiology · 2022
    Article
  4. Article
  5. Article
  6. Biocontrol ofInsects · 2021
    Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Detection of the Entomopathogenic FungusFrontiers in microbiology · 2018
    Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

L CanforaCouncil for Agricultural Research and Economics - Research Centre for the Soil-Plant System, Via della Navicella 2-4, 00184 Rome, Italy.
E MalusàCouncil for Agricultural Research and Economics - Research Centre for the Soil-Plant System, Via della Navicella 2-4, 00184 Rome, Italy.
C TkaczukDepartment of Plant Protection and Breeding, Siedlce University of Natural Sciences and Humanities, Prusa 14 Street, 08-110 Siedlce, Poland.
M TartanusResearch Institute of Horticulture, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland.
B H ŁabanowskaResearch Institute of Horticulture, ul. Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland.
F PinzariCouncil for Agricultural Research and Economics - Research Centre for the Soil-Plant System, Via della Navicella 2-4, 00184 Rome, Italy.
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria · ITInstytut Ogrodnictwa · PLNatural History Museum · GBUniversity of Siedlce · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A culture independent method based on qPCR was developed for the detection and quantification of two fungal inoculants in soil. The aim was to adapt a genotyping approach based on SSR (Simple Sequence Repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. Two entomopathogenic fungi, Beauveria bassiana and B. brongniartii, were traced and quantified in soil samples obtained from field trials. These two fungal species were used as biological agents in Poland to control Melolontha melolontha (European cockchafer), whose larvae live in soil menacing horticultural crops. Specificity of SSR markers was verified using controls consisting of: i) soil samples containing fungal spores of B. bassiana and B. brongniartii in known dilutions; ii) the DNA of the fungal microorganisms; iii) soil samples singly inoculated with each fungus species. An initial evaluation of the protocol was performed with analyses of soil DNA and mycelial DNA. Further, the simultaneous detection and quantification of B. bassiana and B. brongniartii in soil was achieved in field samples after application of the bio-inoculants. The protocol can be considered as a relatively low cost solution for the detection, identification and traceability of fungal bio-inoculants in soil.

Indexed as

Soil MicrobiologyAnimalsBeauveriaDNA, FungalMalusMicrosatellite RepeatsMyceliumMycological Typing TechniquesReproducibility of ResultsRhizosphereSoilSpecies SpecificityDNA, FungalSoil

Identifiers

PMID26975931
PMCPMC4791642
OpenAlexW2301552101

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.