ArticleScientific reports2016
Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- Expansion microscopy applied to mono- and dual-species biofilms.NPJ biofilms and microbiomes · 2023Article
- Expansion Microscopy of Bacillus subtilis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision.Plant methods · 2022Article
- Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).eLife · 2022Article
- Deep Learning-Enabled Resolution-Enhancement in Mini- and Regular Microscopy for Biomedical Imaging.Sensors and actuators. A, Physical · 2021Article
- Ultrastructure expansion microscopy inOpen biology · 2021Article
- Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification.Molecular biology of the cell · 2020Article
- Fluorescence Photobleaching as an Intrinsic Tool to Quantify the 3D Expansion Factor of Biological Samples in Expansion Microscopy.ACS omega · 2020Article
- Mechanically resolved imaging of bacteria using expansion microscopy.PLoS biology · 2019Article
- Nanoscale imaging of E. coli cells by expansion microscopy.Discoveries (Craiova, Romania) · 2019Article
- Expansion microscopy: enabling single cell analysis in intact biological systems.The FEBS journal · 2019Review
- Three-Dimensional and Chemical Mapping of Intracellular Signaling Nanodomains in Health and Disease with Enhanced Expansion Microscopy.ACS nano · 2019Article
- A miniaturized optical tomography platform for volumetric imaging of engineered living systems.Lab on a chip · 2019Article
- Gut-microbiota-on-a-chip: an enabling field for physiological research.Microphysiological systems · 2018Article
- RNA Localization in Bacteria.Microbiology spectrum · 2018Review
- The State of the NIH BRAIN Initiative.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Expansion microscopy: development and neuroscience applications.Current opinion in neurobiology · 2018Review
- Concepts in Light Microscopy of Viruses.Viruses · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
Abstract
To date, much effort has been expended on making high-performance microscopes through better instrumentation. Recently, it was discovered that physical magnification of specimens was possible, through a technique called expansion microscopy (ExM), raising the question of whether physical magnification, coupled to inexpensive optics, could together match the performance of high-end optical equipment, at a tiny fraction of the price. Here we show that such "hybrid microscopy" methods--combining physical and optical magnifications--can indeed achieve high performance at low cost. By physically magnifying objects, then imaging them on cheap miniature fluorescence microscopes ("mini-microscopes"), it is possible to image at a resolution comparable to that previously attainable only with benchtop microscopes that present costs orders of magnitude higher. We believe that this unprecedented hybrid technology that combines expansion microscopy, based on physical magnification, and mini-microscopy, relying on conventional optics--a process we refer to as Expansion Mini-Microscopy (ExMM)--is a highly promising alternative method for performing cost-effective, high-resolution imaging of biological samples. With further advancement of the technology, we believe that ExMM will find widespread applications for high-resolution imaging particularly in research and healthcare scenarios in undeveloped countries or remote places.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.