Evidence map›Paper›PMID 26975733›Full record

ArticleTransgenic research2016

The C-terminus of DSX(F5) protein acts as a novel regulatory domain in Bombyx mori.

Jianping Duan, Xianxin Meng, Sanyuan Ma, Feng Wang, Huozhen Guo, Liying Zhang, Ping Zhao, Yunchao Kan, Lunguang Yao, Qingyou Xia

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Article in Transgenic research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Jianping DuanState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Xianxin MengHenan Provincial Key Laboratory of Funiu Mountain Insect Biology and School of Education, Nanyang Normal University, Nanyang, 473061, People's Republic of China.
Sanyuan MaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Feng WangState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Huozhen GuoState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Liying ZhangState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Ping ZhaoState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China.
Yunchao KanHenan Provincial Key Laboratory of Funiu Mountain Insect Biology and School of Education, Nanyang Normal University, Nanyang, 473061, People's Republic of China.
Lunguang YaoHenan Provincial Key Laboratory of Funiu Mountain Insect Biology and School of Education, Nanyang Normal University, Nanyang, 473061, People's Republic of China.
Qingyou XiaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China. xiaqy@swu.edu.cn.
State Key Laboratory of Silkworm Genomic BiologyNanyang Normal University · CNSouthwest University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The doublesex gene regulates the somatic sexual development of Bombyx mori by alternatively splicing into sex-specific splice forms. In our previous study, the splice form Bmdsx (F7) , which encodes the BmDSX(F5) protein, was found to be expressed in a female-specific manner and to contain a novel C-terminus. In this study, we aimed to investigate the role of this C-terminus. Two transgenic lines, L1 and L2, were constructed to ectopically express Bmdsx (F7) in males. Phenotype and W chromosome-specific polymerase chain reaction (PCR) analysis showed that developmental abnormalities and sex reversal did not occur. Moreover, the sex ratio was also normal. Quantitative PCR revealed that the expression levels of SP1 and Vg were upregulated in the fat body of transgenic males. Additionally, the expression level of PBP was downregulated in the antenna of transgenic males. The results suggested that the C-terminus of BmDSX(F5) functioned as a regulatory domain during regulation of downstream target gene expression and that BmDSX(F5) participated in the sexual development of somatic cells together with other DSX proteins in B. mori.

Indexed as

Gene Expression Regulation, DevelopmentalAnimalsAnimals, Genetically ModifiedBombyxFemaleInsect ProteinsMaleProtein DomainsSex DifferentiationInsect ProteinsBombyx moriC-terminusDoublesex geneTarget genesTransgene

Identifiers

PMID26975733
OpenAlexW2300354337

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.