ArticleJournal of the American Society of Nephrology : JASN2016
Validation of a Three-Dimensional Method for Counting and Sizing Podocytes in Whole Glomeruli.
Article in Journal of the American Society of Nephrology : JASN, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 67 citations in OpenAlex.
- Glomage: A Multimodal Platform for High-Content Morphological and RNA Profiling of Glomeruli in Zebrafish and Mouse Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Mechanisms of Podocyte Injury in Kidney Allografts.Kidney360 · 2026Review
- Podometrics as a translational platform for kidney health and disease: Oshima award address 2025.Clinical and experimental nephrology · 2026Review
- Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.Seminars in nephrology · 2026Review
- Super-resolved optical imaging, reconstruction, and spatial analysis of whole mouse glomeruli via the Glomerulus Mapping and Analysis Pipeline.Kidney international · 2025Article
- The Life of a Kidney Podocyte.Acta physiologica (Oxford, England) · 2025Review
- Three-dimensional reconstruction of fetal rhesus macaque kidneys at single-cell resolution reveals complex inter-relation of structures.bioRxiv : the preprint server for biology · 2024Article
- Podocyte injury at young age causes premature senescence and worsens glomerular aging.American journal of physiology. Renal physiology · 2024Article
- Podocyte number and glomerulosclerosis indices are associated with the response to therapy for primary focal segmental glomerulosclerosis.Frontiers in medicine · 2024Article
- Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue.PNAS nexus · 2024Article
- Spatial transcriptomics using multiplexed deterministic barcoding in tissue.Nature communications · 2023Article
- Nephrons, podocytes and chronic kidney disease: Strategic antihypertensive therapy for renoprotection.Hypertension research : official journal of the Japanese Society of Hypertension · 2023Review
- Quantifying Podocyte Number in a Small Sample Size of Glomeruli with CUBIC to Evaluate Podocyte Depletion of db/db Mice.Journal of diabetes research · 2023Article
- Nephron deficit and low podocyte density increase risk of albuminuria and glomerulosclerosis in a model of diabetes.Physiological reports · 2023Article
- Confocal laser scanning microscopy (CLSM) as a new tool for morphological characterisation of both newly collected and museum voucher specimens of the Trypanorhyncha Diesing, 1863 (Platyhelminthes: Cestoda).Folia parasitologica · 2022Article
- PodoCount: A Robust, Fully Automated, Whole-Slide Podocyte Quantification Tool.Kidney international reports · 2022Article
- The ability of remaining glomerular podocytes to adapt to the loss of their neighbours decreases with age.Cell and tissue research · 2022Article
- Mechanisms of podocyte injury and implications for diabetic nephropathy.Clinical science (London, England : 1979) · 2022Article
- Tissue Cytometry With Machine Learning in Kidney: From Small Specimens to Big Data.Frontiers in physiology · 2022Review
- Three-Dimensional Visualization With Tissue Clearing Uncovers Dynamic Alterations of Renal Resident Mononuclear Phagocytes After Acute Kidney Injury.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Podocyte depletion is sufficient for the development of numerous glomerular diseases and can be absolute (loss of podocytes) or relative (reduced number of podocytes per volume of glomerulus). Commonly used methods to quantify podocyte depletion introduce bias, whereas gold standard stereologic methodologies are time consuming and impractical. We developed a novel approach for assessing podocyte depletion in whole glomeruli that combines immunofluorescence, optical clearing, confocal microscopy, and three-dimensional analysis. We validated this method in a transgenic mouse model of selective podocyte depletion, in which we determined dose-dependent alterations in several quantitative indices of podocyte depletion. This new approach provides a quantitative tool for the comprehensive and time-efficient analysis of podocyte depletion in whole glomeruli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.