ArticleGenome biology2016
Spatiotemporal variation of mammalian protein complex stoichiometries.
Article in Genome biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
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- Gene Dosage Sensitivity and Human Genetic Diseases.Journal of inherited metabolic disease · 2025Review
- Circadian regulation of macromolecular complex turnover and proteome renewal.The EMBO journal · 2024Article
- Functional and spatial proteomics profiling reveals intra- and intercellular signaling crosstalk in colorectal cancer.iScience · 2023Article
- Cryo-EM of a heterogeneous biochemical fraction elucidates multiple protein complexes from a multicellular thermophilic eukaryote.Journal of structural biology: X · 2023Article
- Uncoupled evolution of the Polycomb system and deep origin of non-canonical PRC1.Communications biology · 2023Article
- Deep thermal profiling for detection of functional proteoform groups.Nature chemical biology · 2023Article
- Global and tissue-specific aging effects on murine proteomes.Cell reports · 2023Article
- Genetic dissection of the pluripotent proteome through multi-omics data integration.Cell genomics · 2023Article
- An Assessment of Quaternary Structure Functionality in Homomer Protein Complexes.Molecular biology and evolution · 2023Article
- ProInfer: An interpretable protein inference tool leveraging on biological networks.PLoS computational biology · 2023Article
- Recognition of the CCT5 di-Glu degron by CRL4The EMBO journal · 2023Article
- Integration of protein context improves protein-based COVID-19 patient stratification.Clinical proteomics · 2022Article
- Conserved exchange of paralog proteins during neuronal differentiation.Life science alliance · 2022Article
- A Nuclear Belt Fastens on Neural Cell Fate.Cells · 2022Review
- Genome-wide transcript and protein analysis highlights the role of protein homeostasis in the aging mouse heart.Genome research · 2022Article
- Structure, Maintenance, and Regulation of Nuclear Pore Complexes: The Gatekeepers of the Eukaryotic Genome.Cold Spring Harbor perspectives in biology · 2022Review
- Regulation of protein abundance in genetically diverse mouse populations.Cell genomics · 2021Article
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9 authors.
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Abstract
backgroundRecent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at the protein level.
resultsWe used measurements of protein abundance across 11 cell types and five temporal states to analyze the co-expression and the compositional variations of 182 well-characterized protein complexes. We show that although the abundance of protein complex members is generally co-regulated, a considerable fraction of all investigated protein complexes is subject to stoichiometric changes. Compositional variation is most frequently seen in complexes involved in chromatin regulation and cellular transport, and often involves paralog switching as a mechanism for the regulation of complex stoichiometry. We demonstrate that compositional signatures of variable protein complexes have discriminative power beyond individual cell states and can distinguish cancer cells from healthy ones.
conclusionsOur work demonstrates that many protein complexes contain variable members that cause distinct stoichometries and functionally fine-tune complexes spatiotemporally. Only a fraction of these compositional variations is mediated by changes in transcription and other mechanisms regulating protein abundance contribute to determine protein complex stoichiometries. Our work highlights the superior power of proteome profiles to study protein complexes and their variants across cell states.
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