ArticleScientific reports2016
Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 36 citations in OpenAlex.
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- Ortholog Analysis and Transformation of Glycoside Hydrolase Genes in Hyperthermophilic ArchaealInternational journal of molecular sciences · 2025Article
- Regulation of gene expression under high hydrostatic pressure: the versatile role of the master regulator SurR in energy metabolism.Frontiers in microbiology · 2025Article
- Redirecting electron flow in Acetobacterium woodii enables growth on CO and improves growth on formate.Nature communications · 2024Article
- An archaeal transcription factor EnfR with a novel 'eighth note' fold controls hydrogen production of a hyperthermophilic archaeon Thermococcus onnurineus NA1.Nucleic acids research · 2023Article
- The extremophileFrontiers in microbiology · 2023Article
- A Novel NADP-Dependent Formate Dehydrogenase From the Hyperthermophilic ArchaeonFrontiers in microbiology · 2022Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Biohydrogen production of obligate anaerobic archaeonBiotechnology for biofuels · 2019Article
- Adaptive evolution of a hyperthermophilic archaeon pinpoints a formate transporter as a critical factor for the growth enhancement on formate.Scientific reports · 2017Article
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
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Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H2 on carbon monoxide (CO) and its H2 production rates have been improved through metabolic engineering. In this study, we applied adaptive evolution to enhance H2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H2 productivity at high CO flow rates using synthesis gas produced from coal gasification.
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