ArticleScience advances2016
A three-dimensional culture system recapitulates placental syncytiotrophoblast development and microbial resistance.
Article in Science advances, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed.
- Gene regulation at the early human maternal-fetal interface.Nature reviews. Genetics · 2026Review
- m6A-Driven Pexophagy Triggers Placental Ferroptosis to Impair Fetal Growth Upon Environmental Stress.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advantages, limitations, and innovative considerations for established and emerging models of human placental syncytiotrophoblast.Human reproduction update · 2026Review
- Two classes of syncytiotrophoblast nuclei form following BAP-driven human pluripotent stem cell differentiation.Placenta · 2025Article
- Dynamic Proteome Landscape During Preimplantation Human Embryo Development and Trophectoderm Stem Cell-Differentiation.Proteomics · 2025Article
- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Modeling human placental biology: a review of organoid technologies.Frontiers in cell and developmental biology · 2025Review
- The Interplay of Molecular Factors and Morphology in Human Placental Development and Implantation.Biomedicines · 2024Review
- Modelling the maternal-fetal interface: An in vitro approach to investigate nutrient and drug transport across the human placenta.Journal of cellular and molecular medicine · 2024Article
- Trophoblast Organoids: Capturing the Complexity of Early Placental Development In Vitro.Organoids · 2024Article
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- Controlling placental spheroid growth and phenotype using engineered synthetic hydrogel matrices.Biomaterials science · 2024Article
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- Modeling immunity in microphysiological systems.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- A comprehensive review of human trophoblast fusion models: recent developments and challenges.Cell death discovery · 2023Review
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- Modeling the human placental barrier to understandFrontiers in cellular and infection microbiology · 2023Review
- The transcriptome landscape of 3D-cultured placental trophoblasts reveals activation of TLR2 and TLR3/7 in response to lowFrontiers in microbiology · 2023Article
- Application of a JEG-3 organoid model to study HLA-G function in the trophoblast.Frontiers in immunology · 2023Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
In eutherians, the placenta acts as a barrier and conduit at the maternal-fetal interface. Syncytiotrophoblasts, the multinucleated cells that cover the placental villous tree surfaces of the human placenta, are directly bathed in maternal blood and are formed by the fusion of progenitor cytotrophoblasts that underlie them. Despite their crucial role in fetal protection, many of the events that govern trophoblast fusion and protection from microbial infection are unknown. We describe a three-dimensional (3D)-based culture model using human JEG-3 trophoblast cells that develop syncytiotrophoblast phenotypes when cocultured with human microvascular endothelial cells. JEG-3 cells cultured in this system exhibit enhanced fusogenic activity and morphological and secretory activities strikingly similar to those of primary human syncytiotrophoblasts. RNASeq analyses extend the observed functional similarities to the transcriptome, where we observed significant overlap between syncytiotrophoblast-specific genes and 3D JEG-3 cultures. Furthermore, JEG-3 cells cultured in 3D are resistant to infection by viruses and Toxoplasma gondii, which mimics the high resistance of syncytiotrophoblasts to microbial infections in vivo. Given that this system is genetically manipulatable, it provides a new platform to dissect the mechanisms involved in syncytiotrophoblast development and microbial resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.