ArticleOptica2015
Correcting field-dependent aberrations with nanoscale accuracy in three-dimensional single-molecule localization microscopy.
Article in Optica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed.
- Long-Axial-Range Double-Helix Point Spread Functions for 3D Volumetric Super-Resolution Imaging.The journal of physical chemistry. B · 2024Article
- One-dimensional photonic crystal enhancing spin-to-orbital angular momentum conversion for single-particle tracking.Light, science & applications · 2024Article
- Image restoration model for microscopic defocused images based on blurring kernel guidance.Heliyon · 2024Article
- Traceable localization enables accurate integration of quantum emitters and photonic structures with high yield.Optica quantum · 2024Article
- Large-FOV 3D localization microscopy by spatially variant point spread function generation.Science advances · 2024Article
- Deep-blur: Blind identification and deblurring with convolutional neural networks.Biological imaging · 2024Article
- Multiplexed and Millimeter-Scale Fluorescence Nanoscopy of Cells and Tissue Sections via Prism-Illumination and Microfluidics-Enhanced DNA-PAINT.Chemical & biomedical imaging · 2023Article
- Evaluating the resolution of conventional optical microscopes through point spread function measurement.iScience · 2023Review
- Article
- Simple, Economic, and Robust Rail-Based Setup for Super-Resolution Localization Microscopy.The journal of physical chemistry. A · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation.Nature communications · 2022Article
- Sub-picoliter Traceability of Microdroplet Gravimetry and Microscopy.Analytical chemistry · 2022Article
- Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.Nanophotonics (Berlin, Germany) · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
- Adaptive optics in super-resolution microscopy.Biophysics reports · 2021Article
- Accurate localization microscopy by intrinsic aberration calibration.Nature communications · 2021Article
- Subcellular three-dimensional imaging deep through multicellular thick samples by structured illumination microscopy and adaptive optics.Nature communications · 2021Article
- Molecular-scale axial localization by repetitive optical selective exposure.Nature methods · 2021Article
- Microscope calibration protocol for single-molecule microscopy.Optics express · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The localization of single fluorescent molecules enables the imaging of molecular structure and dynamics with subdiffraction precision and can be extended to three dimensions using point spread function (PSF) engineering. However, the nanoscale accuracy of localization throughout a 3D single-molecule microscope's field of view has not yet been rigorously examined. By using regularly spaced subdiffraction apertures filled with fluorescent dyes, we reveal field-dependent aberrations as large as 50-100 nm and show that they can be corrected to less than 25 nm over an extended 3D focal volume. We demonstrate the applicability of this technique for two engineered PSFs, the double-helix PSF and the astigmatic PSF. We expect these results to be broadly applicable to 3D single-molecule tracking and superresolution methods demanding high accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.