ArticleFrontiers in microbiology2016
Fate of Viable but Non-culturable Listeria monocytogenes in Pig Manure Microcosms.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Exploring the prevalence and antibiotic resistance of Listeria monocytogenes in diverse food commodities across Sikkim, India.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Occurrence and Multidrug Resistance in Strains ofMicroorganisms · 2023Article
- An Exploration ofFoods (Basel, Switzerland) · 2022Review
- Occurrence and Diversity of Listeria monocytogenes Isolated from Two Pig Manure Treatment Plants in France.Microbes and environments · 2022Article
- The Viable But Non-Culturable State ofFrontiers in cellular and infection microbiology · 2022Review
- Detection and Potential Virulence of Viable but Non-Culturable (VBNC)Microorganisms · 2021Review
- Benzalkonium Chloride Induces a VBNC State inMicroorganisms · 2020Article
- Spiking a Silty-Sand Reference Soil with Bacterial DNA: Limits and Pitfalls in the Discrimination of Live and Dead Cells When Applying Ethidium Monoazide (EMA) Treatment.Current microbiology · 2019Article
- Article
- Transcriptomic Analysis of the Adaptation ofFrontiers in microbiology · 2017Article
- Persistence and Potential Viable but Non-culturable State of Pathogenic Bacteria during Storage of Digestates from Agricultural Biogas Plants.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fate of two strains of Listeria monocytogenes and their ability to become viable but non-culturable (VBNC) was investigated in microcosms containing piggery effluents (two raw manures and two biologically treated manures) stored for 2 months at 8 and 20°C. Levels of L. monocytogenes were estimated using the culture method, qPCR, and propidium monoazide treatment combined with qPCR (qPCRPMA). The chemical composition and the microbial community structure of the manures were also analyzed. The strains showed similar decline rates and persisted up to 63 days. At day zero, the percentage of VBNC cells among viable cells was higher in raw manures (81.5-94.8%) than in treated manures (67.8-79.2%). The changes in their proportion over time depended on the temperature and on the type of effluent: the biggest increase was observed in treated manures at 20°C and the smallest increase in raw manures at 8°C. The chemical parameters had no influence on the behavior of the strains, but decrease of the persistence of viable cells was associated with an increase in the microbial richness of the manures. This study demonstrated that storing manure altered the culturability of L. monocytogenes, which rapidly entered the VBNC state, and underlines the importance of including VBNC cells when estimating the persistence of the pathogens in farm effluents.
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