ArticleJournal of virological methods2016
Real-time PCR for differential quantification of CVI988 vaccine virus and virulent strains of Marek's disease virus.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genome-wide analyses of an avian herpesvirus identify 10 loci associated with tumorigenicity and vaccine escape.Science advances · 2026Article
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- High-fidelity long-read sequencing of an avian herpesvirus reveals extensive intrapopulation diversity in tandem repeat regions.PLoS pathogens · 2025Article
- Role of the multiple telomeric repeat arrays in integration, persistence, and efficacy of the commercial CVI988 vaccine.mSphere · 2025Article
- TheViruses · 2024Article
- Comparative analysis of multiple consensus genomes of the same strain of Marek's disease virus reveals intrastrain variation.Virus evolution · 2024Article
- Regulation of Wnt/β-catenin signaling by Marek's disease virusFrontiers in microbiology · 2024Article
- Development of a one-run real-time PCR detection system for pathogens associated with poultry infectious diseases.The Journal of veterinary medical science · 2023Article
- A quadruplex real-time PCR assay combined with a conventional PCR for the differential detection of Marek's disease virus vaccines and field strains.Frontiers in veterinary science · 2023Article
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- Rapid, Sensitive, and Species-Specific Detection of Conventional and Recombinant Herpesvirus of Turkeys Vaccines Using Loop-Mediated Isothermal Amplification Coupled With a Lateral Flow Device Readout.Frontiers in veterinary science · 2022Article
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- Article
- Marek's disease vaccines-induced differential expression of known and novel microRNAs in primary lymphoid organ bursae of White Leghorn.Veterinary research · 2020Article
- Biotic concerns in generating molecular diagnosis matrixes for 4 avian viruses with emphasis on Marek's disease virus.Journal of virological methods · 2019Review
- The Transcriptional Landscape of Marek's Disease Virus in Primary Chicken B Cells Reveals Novel Splice Variants and Genes.Viruses · 2019Article
- Molecular epidemiology of Marek's disease virus in central Pennsylvania, USA.Virus evolution · 2019Article
- Characterization of Marek's disease virus and phylogenetic analyses ofVirusdisease · 2018Article
- Modeling Marek's disease virus transmission: A framework for evaluating the impact of farming practices and evolution.Epidemics · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
CVI988/Rispens vaccine, the 'gold standard' vaccine against Marek's disease in poultry, is not easily distinguishable from virulent strains of Marek's disease herpesvirus (MDV). Accurate differential measurement of CVI988 and virulent MDV is commercially important to confirm successful vaccination, to diagnose Marek's disease, and to investigate causes of vaccine failure. A real-time quantitative PCR assay to distinguish CVI988 and virulent MDV based on a consistent single nucleotide polymorphism in the pp38 gene, was developed, optimised and validated using common primers to amplify both viruses, but differential detection of PCR products using two short probes specific for either CVI988 or virulent MDV. Both probes showed perfect specificity for three commercial preparations of CVI988 and 12 virulent MDV strains. Validation against BAC-sequence-specific and US2-sequence-specific q-PCR, on spleen samples from experimental chickens co-infected with BAC-cloned pCVI988 and wild-type virulent MDV, demonstrated that CVI988 and virulent MDV could be quantified very accurately. The assay was then used to follow kinetics of replication of commercial CVI988 and virulent MDV in feather tips and blood of vaccinated and challenged experimental chickens. The assay is a great improvement in enabling accurate differential quantification of CVI988 and virulent MDV over a biologically relevant range of virus levels.
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