ArticleJournal of neuroscience methods2016
Determining synaptic parameters using high-frequency activation.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
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- Synapse-Specific Defects in Synaptic Transmission in the Cerebellum of W246G Mutant ELOVL4 Rats-a Model of Human SCA34.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.eLife · 2023Article
- SNARE Proteins in Synaptic Vesicle Fusion.Advances in neurobiology · 2023Article
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- Presynaptic Rac1 controls synaptic strength through the regulation of synaptic vesicle priming.eLife · 2022Article
- Alix is required for activity-dependent bulk endocytosis at brain synapses.PLoS biology · 2022Article
- Structural and Functional Plasticity in the Dorsolateral Geniculate Nucleus of Mice following Bilateral Enucleation.Neuroscience · 2022Article
- Adolescent social isolation induced alterations in nucleus accumbens glutamate signalling.Addiction biology · 2022Article
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- Introduction of synaptotagmin 7 promotes facilitation at the climbing fiber to Purkinje cell synapse.Cell reports · 2021Article
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- The Property-Based Practical Applications and Solutions of Genetically Encoded Acetylcholine and Monoamine Sensors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Review
- Diverse roles of Synaptotagmin-7 in regulating vesicle fusion.Current opinion in neurobiology · 2020Review
- Synapse elimination activates a coordinated homeostatic presynaptic response in an autaptic circuit.Communications biology · 2020Article
- Role of GluA3 AMPA Receptor Subunits in the Presynaptic and Postsynaptic Maturation of Synaptic Transmission and Plasticity of Endbulb-Bushy Cell Synapses in the Cochlear Nucleus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Short-term plasticity in glomerular inhibitory circuits shapes olfactory bulb output.Journal of neurophysiology · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe specific properties of a synapse determine how neuronal activity evokes neurotransmitter release. Evaluating changes in synaptic properties during sustained activity is essential to understanding how genetic manipulations and neuromodulators regulate neurotransmitter release. Analyses of postsynaptic responses to high-frequency stimulation have provided estimates of the size of the readily-releasable pool (RRP) of vesicles (N0) and the probability of vesicular release (p) at multiple synapses. NEW
methodHere, we introduce a model-based approach at the calyx of Held synapse in which depletion and the rate of replenishment (R) determine the number of available vesicles, and facilitation leads to a use-dependent increase in p when initial p is low.
resultsWhen p is high and R is low, we find excellent agreement between estimates based on all three methods and the model. However, when p is low or when significant replenishment occurs between stimuli, estimates of different methods diverge, and model estimates are between the extreme estimates provided by the other approaches. COMPARISON WITH OTHER
methodsWe compare our model-based approach to three other approaches that rely on different simplifying assumptions. Our findings suggest that our model provides a better estimate of N0 and p than previously-established methods, likely due to inaccurate assumptions about replenishment. More generally, our findings suggest that approaches commonly used to estimate N0 and p at other synapses are often applied under experimental conditions that yield inaccurate estimates.
conclusionsCareful application of appropriate methods can greatly improve estimates of synaptic parameters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.