ArticleForensic science, medicine, and pathology2016
Novel identification of biofluids using a multiplex methylation-specific PCR combined with single-base extension system.
Article in Forensic science, medicine, and pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Multiplex methylation-specific PCR for distinguishing semen, saliva, and blood.International journal of legal medicine · 2026Article
- Recent advances in the identification of vaginal fluid for forensic applications.International journal of legal medicine · 2026Review
- Pitfalls in body fluid identification - age independent DNA methylation markers for vaginal secretions and menstrual blood in sexual assaults.International journal of legal medicine · 2026Article
- Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.International journal of legal medicine · 2026Article
- Identification of menstrual blood and vaginal fluid using a 4-plex MSRE-PCR system applicable to alleged sexual assault cases.International journal of legal medicine · 2025Article
- Application of a new composite genetic marker semen-specific methylation-microhaplotype in the analysis of semen-vaginal fluid mixtures.Royal Society open science · 2025Article
- Development of a novel panel for blood identification based on blood-specific CpG-linked SNP markers.International journal of legal medicine · 2024Article
- Expanding the scope of methylation-sensitive restriction enzyme (MSRE) PCR for forensic identification of body fluids through the novel use of methylation-dependent restriction enzymes (MDRE) and the combination of autosomal and Y-chromosomal markers.International journal of legal medicine · 2024Article
- More than just blood, saliva, or sperm-setup of a workflow for body fluid identification by DNA methylation analysis.International journal of legal medicine · 2023Article
- Knife wound or nosebleed-where does the blood at the crime scene come from?International journal of legal medicine · 2023Article
- Accurate age estimation from blood samples of Han Chinese individuals using eight high-performance age-related CpG sites.International journal of legal medicine · 2022Article
- On the Identification of Body Fluids and Tissues: A Crucial Link in the Investigation and Solution of Crime.Genes · 2021Review
- Development of a multiplex methylation-sensitive restriction enzyme-based SNP typing system for deconvolution of semen-containing mixtures.International journal of legal medicine · 2021Article
- Identification of spermatozoa using a novel 3-plex MSRE-PCR assay for forensic examination of sexual assaults.International journal of legal medicine · 2020Article
- Characterization of DNA methylation-based markers for human body fluid identification in forensics: a critical review.International journal of legal medicine · 2020Review
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
purposeKnowledge of the composition of complex body fluid mixtures may aid forensic investigations greatly. However, many of the traditional tests are presumptive in nature and can lead to ambiguous results. The aim of this study is to establish a reliable method to identify various biofluids via analysis of their DNA methylation profiles.
methodsA total of eight biofluid-specific methylated markers for saliva, venous blood, vaginal fluids, and semen were isolated from the open database of Infinium HumanMethylation450 BeadChip. These biofluid-specific markers, a control marker to confirm bisulfite conversion, and a gender marker, were combined into a 10-plex methylation-specific PCR single-base-extension (MSP-SBE) system.
resultsAnalysis of 65 DNA samples isolated from venous blood, semen, vaginal fluid, saliva, and menstrual blood that had been treated with bisulfite, resulted in all eight markers detecting the body fluid to which they were designed. Unambiguous body fluid identification occurred from both single sources of body fluids and complex mixtures. A threshold was devised for each marker to minimize the chance of a false inclusion. The efficacy of the assay and application to forensic practice was demonstrated using five non-probative samples from real alleged sexual assault cases. The system unambiguously determined the biofluid types for the non-probative forensic samples that previously resulted in inconclusive or conflicting results using traditional tests.
conclusionsThe results demonstrated the 10-plex MSP-SBE system established in this study is both sensitive and specific when applied to body fluid identification and can be readily adopted into forensic practice.
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