Evidence map›Paper›PMID 26972692›Full record

ArticleInternational journal of legal medicine2016

Detection of a G>C single nucleotide polymorphism within a repetitive DNA sequence by high-resolution DNA melting.

Ulrike Schmidt, Johannes Hulkkonen, Jana Naue

Abstract readComparative Study
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In one paragraph

Article in International journal of legal medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ulrike SchmidtInstitute of Legal Medicine, Freiburg University Medical Center, Albertstr. 9, 79104, Freiburg, Germany. ulrike.schmidt@uniklinik-freiburg.de.
Johannes HulkkonenInstitute of Legal Medicine, Freiburg University Medical Center, Albertstr. 9, 79104, Freiburg, Germany.
Jana NaueInstitute of Legal Medicine, Freiburg University Medical Center, Albertstr. 9, 79104, Freiburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In standard forensic DNA analysis, single base mutations within short tandem repeats (STR) mostly escape detection. In this study, high-resolution DNA melting (HRM) is compared to minisequencing and Sanger sequencing as to determine the most suitable method for detection of a G to C mutation within a repetitive DNA sequence, the STR system DXS10161. It shows an ATG/ATC polymorphism surrounded by a variable number of (TATC) and (ATCT) motifs. Neutral base changes like G:C to C:G result in very low differences in the melting temperature (T m) of the PCR amplicons. By enhanced resolution of fluorescence vs. temperature in HRM, the technique showed to be suitable for detecting a G to C transversion in this repetitive DNA sequence context. Compared to minisequencing, HRM is more time- and cost-effective. Results were confirmed by Sanger sequencing.

Indexed as

Nucleic Acid DenaturationPolymorphism, Single NucleotideFemaleForensic GeneticsGenotypeHumansMaleMicrosatellite RepeatsSequence Analysis, DNAForensic genotypingHigh-resolution melting (HRM)MinisequencingSanger sequencingShort tandem repeat (STR)Single nucleotide polymorphism (SNP)

Identifiers

PMID26972692

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.