ArticleScientific reports2016
Selection of suitable reference genes for assessing gene expression in pearl millet under different abiotic stresses and their combinations.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Identification of Reference Genes and Their Application in Analysis of Ginsenoside Biosynthesis Genes in American Ginseng Under Abiotic Stresses and Hormone Treatments.Plants (Basel, Switzerland) · 2026Article
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- Screening and validation of reference genes of Gynostemma pentaphyllum with different tissues and under different treatment conditions by RT-qPCR analysis.Molecular biology reports · 2025Article
- PgF3H gene enhances drought tolerance in transgenic Arabidopsis by regulating flavonoid biosynthesis and stress response.Plant cell reports · 2025Article
- Comprehensive analysis of housekeeping genes, tissue-specific genes, and dynamic regulation across developmental stages in pearl millet.BMC genomics · 2024Article
- Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.Molecular biology reports · 2024Article
- Bacillus australimaris protect Gloriosa superba L. against Alternaria alternata infestation.World journal of microbiology & biotechnology · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Physiological and Molecular Responses ofPlants (Basel, Switzerland) · 2024Article
- Comprehensive analysis of B3 family genes in pearl millet (Frontiers in plant science · 2024Article
- Selection and validation of reliable reference genes for quantitative real-time PCR in Barnyard millet (Echinochloa spp.) under varied abiotic stress conditions.Scientific reports · 2023Article
- Genetic insights in pearl millet breeding in the genomic era: challenges and prospects.Plant biotechnology reports · 2023Review
- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- Transcriptome-based analysis of key pathways relating to yield formation stage of foxtail millet under different drought stress conditions.Frontiers in plant science · 2022Article
- Identification of HvLRX, a new dehydration and light responsive gene in Tibetan hulless barley (Hordeum vulgare var. nudum).Genes & genomics · 2021Article
- Research on the drought tolerance mechanism of Pennisetum glaucum (L.) in the root during the seedling stage.BMC genomics · 2021Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.Scientific reports · 2021Article
- Identification of suitable reference genes for expression studies in pomegranate under different biotic and abiotic stress conditions.Molecular biology reports · 2021Article
- Selection and validation reference genes for qRT-PCR normalization in different cultivars during fruit ripening and softening of peach (Prunus persica).Scientific reports · 2021Article
- Silver nanoparticles improved the plant growth and reduced the sodium and chlorine accumulation in pearl millet: a life cycle study.Environmental science and pollution research international · 2021Article
1 more citing papers are in PubMed but not listed here.
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Abstract
Pearl millet [Pennisetum glaucum (L.) R. Br.] a widely used grain and forage crop, is grown in areas frequented with one or more abiotic stresses, has superior drought and heat tolerance and considered a model crop for stress tolerance studies. Selection of suitable reference genes for quantification of target stress-responsive gene expression through quantitative real-time (qRT)-PCR is important for elucidating the molecular mechanisms of improved stress tolerance. For precise normalization of gene expression data in pearl millet, ten candidate reference genes were examined in various developmental tissues as well as under different individual abiotic stresses and their combinations at 1 h (early) and 24 h (late) of stress using geNorm, NormFinder and RefFinder algorithms. Our results revealed EF-1α and UBC-E2 as the best reference genes across all samples, the specificity of which was confirmed by assessing the relative expression of a PgAP2 like-ERF gene that suggested use of these two reference genes is sufficient for accurate transcript normalization under different stress conditions. To our knowledge this is the first report on validation of reference genes under different individual and multiple abiotic stresses in pearl millet. The study can further facilitate fastidious discovery of stress-tolerance genes in this important stress-tolerant crop.
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