ArticleScientific reports2016
Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- A Novel Bi-Directional Channel for Nutrient Uptake across Mycobacterial Outer Envelope.Microorganisms · 2024Article
- Identification of Toxic Proteins Encoded by Mycobacteriophage TM4 Using a Next-Generation Sequencing-Based Method.Microbiology spectrum · 2023Article
- Construction of Novel Live Genetically Modified BCG Vaccine Candidates Using Recombineering Tools.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Identification and architecture of a putative secretion tube across mycobacterial outer envelope.Science advances · 2021Article
- Structural insights into the functional divergence of WhiB-like proteins in Mycobacterium tuberculosis.Molecular cell · 2021Article
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- Characterization of a toxin-antitoxin system in Mycobacterium tuberculosis suggests neutralization by phosphorylation as the antitoxicity mechanism.Communications biology · 2020Article
- Article
- Structural basis of non-canonical transcriptional regulation by the σA-bound iron-sulfur protein WhiB1 in M. tuberculosis.Nucleic acids research · 2020Article
- Interference with Pseudomonas aeruginosa Quorum Sensing and Virulence by the MycobacterialInfection and immunity · 2019Article
- A highly efficient in vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineering.Journal of genetics and genomics = Yi chuan xue bao · 2019Article
- MnoSR Is a Bona Fide Two-Component System Involved in Methylotrophic Metabolism in Mycobacterium smegmatis.Applied and environmental microbiology · 2019Article
- Methylotrophy in Mycobacteria: Dissection of the Methanol Metabolism Pathway in Mycobacterium smegmatis.Journal of bacteriology · 2018Article
- CRISPR-Cas12a-Assisted Recombineering in Bacteria.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Research on mycobacterial genetics relies heavily on techniques for directed gene mutation, but genetic studies are often hampered by the difficulty of generating gene deletions in mycobacteria. We developed an efficient and improved deletion system, described here in detail, which can be used to construct multiple unmarked recombinants in mycobacteria. We tested this system by using it to sequentially delete four pairs of toxin-antitoxin genes in Mycobacterium smegmatis.
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Registered trials
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