Evidence map›Paper›PMID 26971664›Full record

ArticleActa biomaterialia2016

Dentin regeneration by stem cells of apical papilla on injectable nanofibrous microspheres and stimulated by controlled BMP-2 release.

Wei Wang, Ming Dang, Zhanpeng Zhang, Jiang Hu, Thomas W Eyster, Longxing Ni, Peter X Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  12. Research progress of dental pulp regeneration treatment.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2024
    Review
  13. Article
  14. Stem Cells and Bone Tissue Engineering.Life (Basel, Switzerland) · 2024
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wei WangDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA; State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Ming DangMacromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI 48109, USA.
Zhanpeng ZhangDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Jiang HuDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
Thomas W EysterDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
Longxing NiState Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, People's Republic of China. Electronic address: Longxing_Ni@hotmail.com.
Peter X MaDepartment of Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mapx@umich.edu.

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
PTH and Calcium Synergy for Craniofacial RegenerationR01DE022327 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MCCAULEY, LAURIE K. · 2012 to 2022
$3.8M
Periodontal Engineering Using Biomimetic Nano ScaffoldsR01DE015384 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MA, PETER X · 2003 to 2011
$2.9M
NIDCR NIH HHS R01 DE015384NIDCR NIH HHS R01 DE022327NIDCR NIH HHS T32 DE007057
6 · The paper itself

Abstract

unlabelledThe aim of this study was to investigate the effects of PLLA nanofibrous microspheres (NF-MS) as a cell delivery carrier in combination with controlled release of BMP-2 from PLGA microspheres on the induction of odontogenic differentiation of human stem cells of apical papilla (SCAP). Injectable NF-MS, which mimic the physical architecture of collagen fibers on the nano scale, were fabricated by combining thermally-induced phase separation techniques with an emulsification process. SCAP cultured in a monolayer or cultured on NF-MS in spinner flasks were treated with 100ng/ml BMP-2 in vitro. Odontogenic differentiation was characterized by measuring alkaline phosphatase activity, odontogenic gene expression levels, calcium content, and dentin sialophosphoprotein accumulation. The results demonstrated that BMP-2 enhanced human SCAP odontogenic differentiation both in monolayer culture and on 3D NF-MS in spinner flask culture in vitro. We also developed and tested a system combining NF-MS with controlled BMP-2 release for dentin regeneration in vivo. The results indicate that controlled release of BMP-2 promoted more mineralization and osteodentin formation compared to a BSA-releasing control in a dose-dependent and time-dependent manner. In summary, the NF-MS combined with controlled release of BMP-2 provides an excellent microenvironment for SCAP to regenerate dentin tissue. STATEMENT OF SIGNIFICANCE: Tooth lesion and loss affect masticatory efficiency, speaking function, facial aesthetics and even psychological health. Current treatments depend on "inert" restorative materials, which do not have the healing capacity and may lead to the failure of the restorations over a long term. The aim of this study was to develop an injectable biomaterial and desired growth factor delivery system to support stem cells for mineralized dental tissue regeneration. The study showed that novel injectable and biodegradable nanofibrous microspheres and controlled release of BMP-2 synergistically induce the odontogenic differentiation of human stem cells from the apical papilla and mineralized tissue regeneration, demonstrating the potential of living dental tissue repair.

Indexed as

Bone Morphogenetic Protein 2MicrospheresStem Cell TransplantationAdolescentAnimalsDelayed-Action PreparationsDentinFemaleHeterograftsHumansMaleMiceMice, NudeNanofibersStem CellsBMP2 protein, humanBone Morphogenetic Protein 2Delayed-Action PreparationsBone morphogenetic protein-2 (BMP-2)Controlled deliveryNanofibrous microspheres (NF-MS)Odontogenic differentiationStem cells of apical papilla (SCAP)

Identifiers

PMID26971664
PMCPMC4846535

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.