ArticleScientific reports2016
Atomic visualization of a flipped-back conformation of bisected glycans bound to specific lectins.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Bisecting βGlcNAc: MA'AT analysis ofThe Journal of biological chemistry · 2026Article
- Review
- Oxr1 and Ncoa7 regulate V-ATPase to achieve optimal pH for glycosylation within the Golgi apparatus and trans-Golgi network.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- BisectingACS chemical biology · 2025Article
- Exploring the Impact of Mitoquinone Supplementation on Glycan Profiles in a Repeated Mild Traumatic Brain Injury Mouse Model.Neurotrauma reports · 2025Article
- Regulation of intracellular activity of N-glycan branching enzymes in mammals.The Journal of biological chemistry · 2024Review
- A glycan biomarker predicts cognitive decline in amyloid- and tau-negative patients.Brain communications · 2024Article
- Discovery of a lectin domain that regulates enzyme activity in mouse N-acetylglucosaminyltransferase-IVa (MGAT4A).Communications biology · 2022Article
- Systematic synthesis of bisectedChemical science · 2022Article
- Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues.Nature communications · 2022Article
- Towards Mapping of the Human Brain N-Glycome with Standardized Graphitic Carbon Chromatography.Biomolecules · 2022Article
- Tissue-Specific Regulation of HNK-1 Biosynthesis by Bisecting GlcNAc.Molecules (Basel, Switzerland) · 2021Article
- Peptide Sequence Mapping around Bisecting GlcNAc-BearingInternational journal of molecular sciences · 2021Article
- Towards structure-focused glycoproteomics.Biochemical Society transactions · 2021Review
- Recent Advances in the Chemical Biology ofMolecules (Basel, Switzerland) · 2021Review
- Are Dietary Lectins Relevant Allergens in Plant Food Allergy?Foods (Basel, Switzerland) · 2020Review
- 3D Structure and Function of Glycosyltransferases Involved inInternational journal of molecular sciences · 2020Review
- The Essential Functions and Detection of Bisecting GlcNAc in Cell Biology.Frontiers in chemistry · 2020Review
- Bisecting GlcNAc Is a General Suppressor of Terminal Modification ofMolecular & cellular proteomics : MCP · 2019Article
- Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycans normally exist as a dynamic equilibrium of several conformations. A fundamental question concerns how such molecules bind lectins despite disadvantageous entropic loss upon binding. Bisected glycan, a glycan possessing bisecting N-acetylglucosamine (GlcNAc), is potentially a good model for investigating conformational dynamics and glycan-lectin interactions, owing to the unique ability of this sugar residue to alter conformer populations and thus modulate the biological activities. Here we analyzed bisected glycan in complex with two unrelated lectins, Calsepa and PHA-E. The crystal structures of the two complexes show a conspicuous flipped back glycan structure (designated 'back-fold' conformation), and solution NMR analysis also provides evidence of 'back-fold' glycan structure. Indeed, statistical conformational analysis of available bisected and non-bisected glycan structures suggests that bisecting GlcNAc restricts the conformations of branched structures. Restriction of glycan flexibility by certain sugar residues may be more common than previously thought and impinges on the mechanism of glycoform-dependent biological functions.
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