Evidence map›Paper›PMID 26971467›Full record

ArticleArchives of biochemistry and biophysics2016

Liver Kinase B1 complex acts as a novel modifier of myofilament function and localizes to the Z-disk in cardiac myocytes.

Samantha M Behunin, Marissa A Lopez-Pier, Rachel M Mayfield, Christiane A Danilo, Yulia Lipovka, Camille Birch, Sarah Lehman, Jil C Tardiff, Carol C Gregorio, John P Konhilas

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of biochemistry and biophysics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Myofilament modulation of contraction.Archives of biochemistry and biophysics · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Samantha M BehuninDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Marissa A Lopez-PierSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA.
Rachel M MayfieldSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA.
Christiane A DaniloDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Yulia LipovkaSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA.
Camille BirchSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA.
Sarah LehmanDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Jil C TardiffSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USA.
Carol C GregorioSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USA.
John P KonhilasDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA. Electronic address: konhilas@arizona.edu.
University of Arizona · US

Funding

VITAMIN AP30CA023074 · NCI · UNIVERSITY OF ARIZONA · PI Teri L Bankhead · 1985 to 2026
$110.2M
SOUTHWEST ENVIRONMENTAL HEALTH SCIENCES CENTERP30ES006694 · NIEHS · UNIVERSITY OF ARIZONA · PI DEAN BILLHEIMER · 1994 to 2026
$36.7M
PHYSIOLOGYT32HL007249 · NHLBI · UNIVERSITY OF ARIZONA · PI Brett A Colson, JOHN P KONHILAS · 1985 to 2026
$12.5M
Integrative Approach to Divergent Remodeling in Thin Filament CardiomyopathiesR01HL075619 · NHLBI · UNIVERSITY OF ARIZONA · PI TARDIFF, JIL C · 2004 to 2025
$8.6M
Deciphering the role of Lmod2 in thin filament length regulation and dilated cardiomyopathyR01HL123078 · NHLBI · UNIVERSITY OF ARIZONA · PI Carol C Gregorio, Shashank Shekhar · 2015 to 2026
$5.6M
Cardiovascular Biomedical Engineering Training GrantT32HL007955 · NHLBI · UNIVERSITY OF ARIZONA · PI BARTON, JENNIFER KEHLET · 2001 to 2020
$5.1M
Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathyR01HL098256 · NHLBI · UNIVERSITY OF ARIZONA · PI KONHILAS, JOHN P · 2010 to 2014
$1.9M
Deciphering the role of the RNA-binding protein, FXR1, in cardiac muscle assemblyR01HL108625 · NHLBI · UNIVERSITY OF ARIZONA · PI GREGORIO, CAROL C · 2012 to 2015
$1.9M
Acquisition of a LTQ Orbitrap XL ETD Hybrid FT Mass SpectrometerS10RR028868 · NCRR · UNIVERSITY OF ARIZONA · PI TSAPRAILIS, GEORGE · 2010 to 2010
$916k
Metabolism, Skeletal Muscle, Cardiac Disease, ExerciseK01AR052840 · NIAMS · UNIVERSITY OF COLORADO · PI KONHILAS, JOHN P · 2006 to 2010
$600k
Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathyK02HL105799 · NHLBI · UNIVERSITY OF ARIZONA · PI KONHILAS, JOHN P · 2011 to 2015
$516k
NCI NIH HHS P30 CA023074NCRR NIH HHS S10 RR028868NHLBI NIH HHS K02 HL105799NHLBI NIH HHS R01 HL075619NHLBI NIH HHS R01 HL098256NHLBI NIH HHS R01 HL108625NHLBI NIH HHS R01 HL123078NHLBI NIH HHS T32 HL007249NHLBI NIH HHS T32 HL007955NIAMS NIH HHS K01 AR052840NIEHS NIH HHS P30 ES006694
6 · The paper itself

Abstract

Contractile perturbations downstream of Ca(2+) binding to troponin C, the so-called sarcomere-controlled mechanisms, represent the earliest indicators of energy remodeling in the diseased heart [1]. Central to cellular energy "sensing" is the adenosine monophosphate-activated kinase (AMPK) pathway, which is known to directly target myofilament proteins and alter contractility [2-6]. We previously showed that the upstream AMPK kinase, LKB1/MO25/STRAD, impacts myofilament function independently of AMPK [5]. Therefore, we hypothesized that the LKB1 complex associated with myofilament proteins and that alterations in energy signaling modulated targeting or localization of the LKB1 complex to the myofilament. Using an integrated strategy of myofilament mechanics, immunoblot analysis, co-immunoprecipitation, mass spectroscopy, and immunofluorescence, we showed that 1) LKB1 and MO25 associated with myofibrillar proteins, 2) cellular energy stress re-distributed AMPK/LKB1 complex proteins within the sarcomere, and 3) the LKB1 complex localized to the Z-Disk and interacted with cytoskeletal and energy-regulating proteins, including vinculin and ATP Synthase (Complex V). These data represent a novel role for LKB1 complex proteins in myofilament function and myocellular "energy" sensing in the heart.

Indexed as

AMP-Activated Protein Kinase KinasesAMP-Activated Protein KinasesAnimalsCalciumCytoskeletonGene Expression RegulationMaleMass SpectrometryMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceMuscle ContractionMyocytes, CardiacMyofibrilsProtein Serine-Threonine KinasesAMP-Activated Protein Kinase KinasesAMP-Activated Protein KinasesCalciumProtein Serine-Threonine KinasesStk11 protein, mouseStk11 protein, ratTroponin CContractilityLiver Kinase B1 (LKB1)MyofibrilMyofilamentVinculinZ-disk

Identifiers

PMID26971467
PMCPMC4899161
OpenAlexW2295230372

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.