Evidence map›Paper›PMID 26971072›Full record

ArticleSchizophrenia research2016

Quantitative mass spectrometry reveals changes in SNAP-25 isoforms in schizophrenia.

Vilte E Barakauskas, Annie Moradian, Alasdair M Barr, Clare L Beasley, Gorazd Rosoklija, J John Mann, Boro Ilievski, Aleksandar Stankov, Andrew J Dwork, Peter Falkai and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Schizophrenia research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.2field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Dynamic Changes of Brain Cilia Transcriptomes across the Human Lifespan.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 8 institutions in 4 countries.

Vilte E BarakauskasBC Mental Health and Addictions Research Institute, Vancouver, BC, Canada; Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada.
Annie MoradianMichael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada.
Alasdair M BarrBC Mental Health and Addictions Research Institute, Vancouver, BC, Canada; Department of Anesthesiology, Pharmacology & Therapeutics, University of British Columbia, Vancouver, BC, Canada.
Clare L BeasleyBC Mental Health and Addictions Research Institute, Vancouver, BC, Canada; Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada.
Gorazd RosoklijaDepartment of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, USA; Macedonian Academy of Sciences and Arts, University "SS. Cyril and Methodius", Skopje, Macedonia; Department of Psychiatry, Columbia University, New York, NY, USA.
J John MannDepartment of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, USA; Department of Psychiatry, Columbia University, New York, NY, USA.
Boro IlievskiDepartment of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, USA; Institute for Pathology, University "SS. Cyril and Methodius", Skopje, Macedonia; Department of Psychiatry, Columbia University, New York, NY, USA.
Aleksandar StankovInstitute of Forensic Medicine, Criminology and Medical Deontology, University "SS. Cyril and Methodius", Skopje, Macedonia.
Andrew J DworkDepartment of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, USA; Department of Pathology and Cell Biology, Columbia University, New York, NY, USA; Department of Psychiatry, Columbia University, New York, NY, USA.
Peter FalkaiDepartment of Psychiatry and Psychotherapy, Ludwig-Maximilians-University, Munich, Germany.
Gregg B MorinMichael Smith Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, BC, Canada; Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada. Electronic address: gmorin@bcgsc.ca.
William G HonerBC Mental Health and Addictions Research Institute, Vancouver, BC, Canada; Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada. Electronic address: william.honer@ubc.ca.
BC Mental Health & Substance Use Services · CACanada's Michael Smith Genome Sciences Centre · CANew York State Psychiatric InstituteUniversity of British Columbia · CAColumbia University · USLudwig-Maximilians-Universität München · DENew York Psychoanalytic Society and Institute · USSs. Cyril and Methodius University in Skopje · MK

Funding

THE NEUROBIOLOGY OF SUICIDAL BEHAVIORP50MH062185 · NIMH · NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC · PI MANN, JOSEPH JOHN · 2000 to 2009
$21.7M
Golgi Studies of SchizophreniaR01MH064168 · NIMH · NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC · PI DWORK, ANDREW J · 2001 to 2013
$4.3M
Cortico-thalamic Glutamate/GABA mRNA in schizophreniaR01MH064673 · NIMH · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI HAROUTUNIAN, VAHRAM · 2003 to 2012
$3.4M
Myelin Pathology in SchizophreniaR01MH060877 · NIMH · NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC · PI DWORK, ANDREW J · 1999 to 2011
$2.4M
NIMH NIH HHS P50 MH062185NIMH NIH HHS R01 MH060877NIMH NIH HHS R01 MH064168NIMH NIH HHS R01 MH064673
6 · The paper itself

Abstract

SNAP-25 and syntaxin are presynaptic terminal SNARE proteins altered in amount and function in schizophrenia. In the ventral caudate, we observed 32% lower SNAP-25 and 26% lower syntaxin, but greater interaction between the two proteins using an in vitro assay. SNAP-25 has two isoforms, SNAP-25A and B, differing by only 9 amino acids, but with different effects on neurotransmission. A quantitative mass spectrometry assay was developed to measure total SNAP-25, and proportions of SNAP-25A and B. The assay had a good linear range (50- to 150-fold) and coefficient of variation (4.5%). We studied ventral caudate samples from patients with schizophrenia (n=15) previously reported to have lower total SNAP-25 than controls (n=13). We confirmed 27% lower total SNAP-25 in schizophrenia, and observed 31% lower SNAP-25A (P=0.002) with 20% lower SNAP-25B amounts (P=0.10). Lower SNAP-25A amount correlated with greater SNAP-25-syntaxin protein-protein interactions (r=-0.41, P=0.03); the level of SNAP-25B did not. Administration of haloperidol or clozapine to rats did not mimic the changes found in schizophrenia. The findings suggest that lower levels of SNAP-25 in schizophrenia may represent a greater effect of the illness on the SNAP-25A isoform. This in turn could contribute to the greater interaction between SNAP25 and syntaxin, and possibly disturb neurotransmission in the illness.

Indexed as

AnimalsAntipsychotic AgentsClozapineCorpus StriatumFemaleHaloperidolHumansImmunoprecipitationMaleMass SpectrometryMiddle AgedProtein IsoformsRats, Sprague-DawleySchizophreniaSynaptosomal-Associated Protein 25Antipsychotic AgentsClozapineHaloperidolProtein IsoformsSNAP25 protein, humanSnap25 protein, ratSynaptosomal-Associated Protein 25Neural plasticityNeurotransmissionPostmortemSNARE proteinsSynapse

Identifiers

PMID26971072
PMCPMC5017887
OpenAlexW2293592800

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.