Evidence map›Paper›PMID 26969721›Full record

ArticlePlant physiology2016

TOPOISOMERASE1α Acts through Two Distinct Mechanisms to Regulate Stele and Columella Stem Cell Maintenance.

Yonghong Zhang, Lanlan Zheng, Jing Han Hong, Ximing Gong, Chun Zhou, José Manuel Pérez-Pérez, Jian Xu

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
7.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Pars Pro Toto: Every Single Cell Matters.Frontiers in plant science · 2021
    Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yonghong ZhangNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).
Lanlan ZhengNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).
Jing Han HongNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).
Ximing GongNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).
Chun ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).
José Manuel Pérez-PérezNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.).ORCID 0000-0003-2848-4919
Jian XuNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China (Y.Z., L.Z., C.Z.);Department of Biological Sciences and NUS Centre for BioImaging Sciences, National University of Singapore, Singapore 117543 (J.H.H., X.G., J.X.); andInstituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain (J.M.P.-P.) dbsxj@nus.edu.sg.ORCID 0000-0001-6194-6316
Huazhong Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

TOPOISOMERASE1 (TOP1), which releases DNA torsional stress generated during replication through its DNA relaxation activity, plays vital roles in animal and plant development. In Arabidopsis (Arabidopsis thaliana), TOP1 is encoded by two paralogous genes (TOP1α and TOP1β), of which TOP1α displays specific developmental functions that are critical for the maintenance of shoot and floral stem cells. Here, we show that maintenance of two different populations of root stem cells is also dependent on TOP1α-specific developmental functions, which are exerted through two distinct novel mechanisms. In the proximal root meristem, the DNA relaxation activity of TOP1α is critical to ensure genome integrity and survival of stele stem cells (SSCs). Loss of TOP1α function triggers DNA double-strand breaks in S-phase SSCs and results in their death, which can be partially reversed by the replenishment of SSCs mediated by ETHYLENE RESPONSE FACTOR115 In the quiescent center and root cap meristem, TOP1α is epistatic to RETINOBLASTOMA-RELATED (RBR) in the maintenance of undifferentiated state and the number of columella stem cells (CSCs). Loss of TOP1α function in either wild-type or RBR RNAi plants leads to differentiation of CSCs, whereas overexpression of TOP1α mimics and further enhances the effect of RBR reduction that increases the number of CSCs Taken together, these findings provide important mechanistic insights into understanding stem cell maintenance in plants.

Indexed as

ArabidopsisArabidopsis ProteinsCell DifferentiationCell SurvivalDNA Topoisomerases, Type IGene Expression Regulation, PlantGenome, PlantMeristemPlant CellsPlant RootsStem CellsTranscription FactorsArabidopsis ProteinsDNA Topoisomerases, Type IERF115 protein, ArabidopsisTOP1alpha protein, ArabidopsisTOP1beta protein, ArabidopsisTranscription Factors

Identifiers

PMID26969721
PMCPMC4854680
OpenAlexW2346009861

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.