ArticleApplied and environmental microbiology2016
Development of a Real-Time PCR for a Sensitive One-Step Coprodiagnosis Allowing both the Identification of Carnivore Feces and the Detection of Toxocara spp. and Echinococcus multilocularis.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 62 citations in OpenAlex.
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- Molecular characterization of Echinococcus species causing human cystic echinococcosis in Algeria: predominance of Echinococcus granulosus sensu stricto.Parasite (Paris, France) · 2026Article
- A novel RFLP-PCR method for the rapid diagnosis of Echinococcus multilocularis and different Echinococcus granulosus sensu lato species from tissue samples.Parasite (Paris, France) · 2026Article
- New evidence from the northern Apennines, Italy, suggests a southward expansion of Echinococcus multilocularis range in Europe.Scientific reports · 2025Article
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- Rapid Discriminative Identification of the Two Predominant Echinococcus Species from Canine Fecal Samples in the Tibetan Region of China by Loop-Mediated Isothermal Amplification-Lateral Flow Dipstick Assay.Tropical medicine and infectious disease · 2024Article
- A walk on the wild side: A review of the epidemiology ofInternational journal for parasitology. Parasites and wildlife · 2023Review
- Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.PLoS neglected tropical diseases · 2023Article
- Field-Crop Soils in Eastern France: Coldspots of Azole-ResistantJournal of fungi (Basel, Switzerland) · 2023Article
- Real-time multiplex PCR for human echinococcosis and differential diagnosis.Parasite (Paris, France) · 2023Article
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- Molecular diagnosis of alveolar echinococcosis in patients based on frozen and formalin-fixed paraffin-embedded tissue samples.Parasite (Paris, France) · 2022Article
- Assessment of the Genetic Diversity ofPathogens (Basel, Switzerland) · 2021Article
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- Investigation of Echinococcus multilocularis in foxes and dogs in Pakistan by detection of copro-DNA.Parasitology research · 2021Article
- Soil contamination by Echinococcus multilocularis in rural and urban vegetable gardens in relation to fox, cat and dog faecal deposits.Parasite (Paris, France) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studying the environmental occurrence of parasites of concern for humans and animals based on coprosamples is an expanding field of work in epidemiology and the ecology of health. Detecting and quantifying Toxocara spp. and Echinococcus multilocularis, two predominant zoonotic helminths circulating in European carnivores, in feces may help to better target measures for prevention. A rapid, sensitive, and one-step quantitative PCR (qPCR) allowing detection of E. multilocularis and Toxocara spp. was developed in the present study, combined with a host fecal test based on the identification of three carnivores (red fox, dog, and cat) involved in the life cycles of these parasites. A total of 68 coprosamples were collected from identified specimens from Vulpes vulpes, Canis lupus familiaris, Canis lupus, Felis silvestris catus, Meles meles, Martes foina, and Martes martes With DNA coprosamples, real-time PCR was performed in duplex with a qPCR inhibitor control specifically designed for this study. All the coprosample host identifications were confirmed by qPCR combined with sequencing, and parasites were detected and confirmed (E. multilocularis in red foxes and Toxocara cati in cats; 16% of samples presented inhibition). By combining parasite detection and quantification, the host fecal test, and a new qPCR inhibitor control, we created a technique with a high sensitivity that may considerably improve environmental studies of pathogens.
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