ArticleApplied and environmental microbiology2016
Isolation of Endohyphal Bacteria from Foliar Ascomycota and In Vitro Establishment of Their Symbiotic Associations.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Endosymbiotic interactions in the fungal kingdom: a framework for progressive endosymbiosis.Frontiers in microbiology · 2026Review
- Artificial Endosymbiosis of Pedobacter sp. DDGJ Boosts the Growth Potential, Stress Resistance and Productivity of Morchella Mushrooms.Microbial biotechnology · 2025Article
- Endofungal bacteria as hidden facilitators of biotic interactions.The ISME journal · 2025Review
- Diversity analysis of endohyphal bacteria in oil-producing fungi inhabiting arid environments.Frontiers in microbiology · 2025Article
- Symbioses between fungi and bacteria: from mechanisms to impacts on biodiversity.Current opinion in microbiology · 2024Review
- Article
- Detecting and characterizing new endofungal bacteria in new hosts:Frontiers in microbiology · 2024Article
- Endosymbiotic bacteria within the nematode-trapping fungusFrontiers in microbiology · 2024Article
- Klebsiella michiganensis: a nitrogen-fixing endohyphal bacterium from Ustilago maydis.AMB Express · 2023Article
- The endohyphal microbiome: current progress and challenges for scaling down integrative multi-omic microbiome research.Microbiome · 2023Article
- The Importance of Microorganisms for Sustainable Agriculture-A Review.Metabolites · 2022Review
- Microbiota manipulation through the secretion of effector proteins is fundamental to the wealth of lifestyles in the fungal kingdom.FEMS microbiology reviews · 2022Review
- Transcriptional Profiles of a Foliar Fungal Endophyte (mSystems · 2022Article
- Bacterial hitchhikers derive benefits from fungal housing.Current biology : CB · 2022Article
- Plant Growth-Promoting Ability of MycorrhizalFrontiers in microbiology · 2022Article
- The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China.Frontiers in microbiology · 2022Article
- Wide distribution of thePlant signaling & behavior · 2021Article
- Deep learning approaches for natural product discovery from plant endophytic microbiomes.Environmental microbiome · 2021Review
- Article
- Diversity and Structure of the Endophytic Bacterial Communities Associated With Three Terrestrial Orchid Species as Revealed by 16S rRNA Gene Metabarcoding.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endohyphal bacteria (EHB) can influence fungal phenotypes and shape the outcomes of plant-fungal interactions. Previous work has suggested that EHB form facultative associations with many foliar fungi in the Ascomycota. These bacteria can be isolated in culture, and fungi can be cured of EHB using antibiotics. Here, we present methods for successfully introducing EHB into axenic mycelia of strains representing two classes of Ascomycota. We first establish in vitro conditions favoring reintroduction of two strains of EHB (Luteibacter sp.) into axenic cultures of their original fungal hosts, focusing on fungi isolated from healthy plant tissue as endophytes: Microdiplodia sp. (Dothideomycetes) and Pestalotiopsis sp. (Sordariomycetes). We then demonstrate that these EHB can be introduced into a novel fungal host under the same conditions, successfully transferring EHB between fungi representing different classes. Finally, we manipulate conditions to optimize reintroduction in a focal EHB-fungal association. We show that EHB infections were initiated and maintained more often under low-nutrient culture conditions and when EHB and fungal hyphae were washed with MgCl2 prior to reassociation. Our study provides new methods for experimental assessment of the effects of EHB on fungal phenotypes and shows how the identity of the fungal host and growth conditions can define the establishment of these widespread and important symbioses.
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