Evidence map›Paper›PMID 26969591›Full record

ArticleNanoscale research letters2016

CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4.

Xinyu Yan, Kan Wang, Wenting Lu, Weijian Qin, Daxiang Cui, Jinghua He

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanoscale research letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 42 citations in OpenAlex.

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  6. Red ZnApplied physics. A, Materials science & processing · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xinyu YanOut-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China.
Kan WangInstitute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. wk_xa@163.com.
Wenting LuOut-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China.
Weijian QinInstitute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China.
Daxiang CuiInstitute of Nano Biomedical and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China. dxcui@sjtu.edu.cn.
Jinghua HeOut-Patient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, 510280, Guangzhou, Guangdong, People's Republic of China. hjh5258@foxmail.com.
Shanghai Jiao Tong University · CNZhujiang Hospital · CN

Funding

Developmental Pathways to Substance Use Across European & Asian American YouthF31AA020700 · NIAAA · UNIVERSITY OF WASHINGTON · PI LUK, JEREMY WING-HEI · 2011 to 2013
$123k
NIAAA NIH HHS F31 AA020700
6 · The paper itself

Abstract

Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.

Indexed as

CA72-4CdSe/ZnS quantum dotsGastric cancerLateral flow strip

Identifiers

PMID26969591
PMCPMC4788655
OpenAlexW2293590389

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.