ArticleApplied microbiology and biotechnology2016
Display of fungal hydrophobin on the Pichia pastoris cell surface and its influence on Candida antarctica lipase B.
Article in Applied microbiology and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes inBioengineering (Basel, Switzerland) · 2025Review
- Immobilization of Lipase B fromInternational journal of molecular sciences · 2022Article
- Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts.Molecules (Basel, Switzerland) · 2022Article
- Cell-surface engineering of yeasts for whole-cell biocatalysts.Bioprocess and biosystems engineering · 2021Review
- Surface display of HFBI and DewA hydrophobins on Saccharomyces cerevisiae modifies tolerance to several adverse conditions and biocatalytic performance.Applied microbiology and biotechnology · 2021Article
- Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris.BMC microbiology · 2020Article
- Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase FromFrontiers in bioengineering and biotechnology · 2020Article
- Role of Hydrophobins in Aspergillus fumigatus.Journal of fungi (Basel, Switzerland) · 2017Article
- Accurate analysis of fusion expression of Pichia pastoris glycosylphosphatidylinositol-modified cell wall proteins.Journal of industrial microbiology & biotechnology · 2017Article
- Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.Journal of structural biology · 2017Article
- Applications of Functional Amyloids from Fungi: Surface Modification by Class I Hydrophobins.Biomolecules · 2017Review
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
To modify the Pichia pastoris cell surface, two classes of hydrophobins, SC3 from Schizophyllum commune and HFBI from Trichoderma reesei, were separately displayed on the cell wall. There was an observable increase in the hydrophobicity of recombinant strains. Candida antarctica lipase B (CALB) was then co-displayed on the modified cells, generating strains GS115/SC3-61/CALB-51 and GS115/HFBI-61/CALB-51. Interestingly, the hydrolytic and synthetic activities of strain GS115/HFBI-61/CALB-51 increased by 37 and 109 %, respectively, but decreased by 26 and 43 %, respectively, in strain GS115/SC3-61/CALB-51 compared with the hydrophobin-minus recombinant strain GS115/CALB-GCW51. The amount of glycerol by-product from the transesterification reaction adsorbed on the cell surface was significantly decreased following hydrophobin modification, removing the glycerol barrier and allowing substrates to access the active sites of lipases. Electron micrographs indicated that the cell wall structures of both recombinant strains appeared altered, including changes to the inner glucan layer and outer mannan layer. These results suggest that the display of hydrophobins can change the surface structure and hydrophobic properties of P. pastoris and affect the catalytic activities of CALB displayed on the surface of P. pastoris cells.
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Registered trials
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