ReviewTrends in biotechnology2016
Redefining Signaling Pathways with an Expanding Single-Cell Toolbox.
Review in Trends in biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale Proteomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Modeling Cellular Signaling Variability Based on Single-Cell Data: The TGFβ-SMAD Signaling Pathway.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Machine learning phenomics (MLP) combining deep learning with time-lapse-microscopy for monitoring colorectal adenocarcinoma cells gene expression and drug-response.Scientific reports · 2022Article
- Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing.Journal of visualized experiments : JoVE · 2021Article
- Facile One-Pot Nanoproteomics for Label-Free Proteome Profiling of 50-1000 Mammalian Cells.Journal of proteome research · 2021Article
- dNEMO: a tool for quantification of mRNA and punctate structures in time-lapse images of single cells.Bioinformatics (Oxford, England) · 2021Article
- Pre-existing Cell States Control Heterogeneity of Both EGFR and CXCR4 Signaling.Cellular and molecular bioengineering · 2021Article
- Isolation of Highly Purified and Viable Retinal Endothelial Cells.Journal of vascular research · 2021Article
- Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo.Developmental cell · 2020Article
- Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells.Journal of visualized experiments : JoVE · 2020Article
- Short-term cellular memory tunes the signaling responses of the chemokine receptor CXCR4.Science signaling · 2019Article
- Nomenclature and heterogeneity: consequences for the use of mesenchymal stem cells in regenerative medicine.Regenerative medicine · 2019Review
- Single-cell quantification of the concentrations and dissociation constants of endogenous proteins.The Journal of biological chemistry · 2019Article
- Biological signal generators: integrating synthetic biology tools andCurrent opinion in systems biology · 2019Article
- Microfluidic platform enables live-cell imaging of signaling and transcription combined with multiplexed secretion measurements in the same single cells.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- Fold-Change Detection of NF-κB at Target Genes with Different Transcript Outputs.Biophysical journal · 2019Article
- Single cell network analysis with a mixture of Nested Effects Models.Bioinformatics (Oxford, England) · 2018Article
- Engineering Approaches to Study Cellular Decision Making.Annual review of biomedical engineering · 2018Review
- Review
- Quantitative profiling of innate immune activation by viral infection in single cells.Integrative biology : quantitative biosciences from nano to macro · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Genetically identical cells respond heterogeneously to uniform environmental stimuli. Consequently, investigating the signaling networks that control these cell responses using 'average' bulk cell measurements can obscure underlying mechanisms and misses information emerging from cell-to-cell variability. Here we review recent technological advances including live-cell fluorescence imaging-based approaches and microfluidic devices that enable measurements of signaling networks, dynamics, and responses in single cells. We discuss how these single-cell tools have uncovered novel mechanistic insights for canonical signaling pathways that control cell proliferation (ERK), DNA-damage responses (p53), and innate immune and stress responses (NF-κB). Future improvements in throughput and multiplexing, analytical pipelines, and in vivo applicability will all significantly expand the biological information gained from single-cell measurements of signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.