Evidence map›Paper›PMID 26968195›Full record

ArticleThe Journal of pharmacology and experimental therapeutics2016

Long-Term Blockade of Cocaine Self-Administration and Locomotor Activation in Rats by an Adenoviral Vector-Delivered Cocaine Hydrolase.

John R Smethells, Natashia Swalve, Stephen Brimijoin, Yang Gao, Robin J Parks, Adam Greer, Marilyn E Carroll

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of pharmacology and experimental therapeutics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

John R SmethellsResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.) jsmethells@gmail.com.
Natashia SwalveResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).
Stephen BrimijoinResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).ORCID http://orcid.org/0000-0003-1838-5423
Yang GaoResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).
Robin J ParksResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).
Adam GreerResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).
Marilyn E CarrollResearch Fellow, Pharmaco-Neuro-Immunology Training Program (J.R.S.) and Department of Biological Science (A.G.), University of Minnesota, Minneapolis, Minnesota; Department of Psychiatry, University of Minnesota Medical School (N.S., M.E.C.), Minneapolis, Minnesota; Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota (S.B., Y.G.); Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada (R.J.P.).
University of Minnesota · US

Funding

Training in PharmacoNeuroImmune Substance Abuse ResearchT32DA007097 · NIDA · UNIVERSITY OF MINNESOTA TWIN CITIES · PI MOLITOR, THOMAS WILLIAM · 1985 to 2021
$13.1M
NIDA NIH HHS T32 DA007097
6 · The paper itself

Abstract

A promising approach in treating cocaine abuse is to metabolize cocaine in the blood using a mutated butyrylcholinesterase (BChE) that functions as a cocaine hydrolase (CocH). In rats, a helper-dependent adenoviral (hdAD) vector-mediated delivery of CocH abolished ongoing cocaine use and cocaine-primed reinstatement of drug-seeking for several months. This enzyme also metabolizes ghrelin, an effect that may be beneficial in maintaining healthy weights. The effect of a single hdAD-CocH vector injection was examined in rats on measures of anxiety, body weight, cocaine self-administration, and cocaine-induced locomotor activity. To examine anxiety, periadolescent rats were tested in an elevated-plus maze. Weight gain was then examined under four rodent diets. Ten months after CocH-injection, adult rats were trained to self-administer cocaine intravenously and, subsequently, cocaine-induced locomotion was tested. Viral gene transfer produced sustained plasma levels of CocH for over 13 months of testing. CocH-treated rats did not differ from controls in measures of anxiety, and only showed a transient reduction in weight gain during the first 3 weeks postinjection. However, CocH-treated rats were insensitive to cocaine. At 10 months postinjection, none of the CocH-treated rats initiated cocaine self-administration, unlike 90% of the control rats. At 13 months postinjection, CocH-treated rats showed no cocaine-induced locomotion, whereas control rats showed a dose-dependent enhancement of locomotion. CocH vector produced a long-term blockade of the rewarding and behavioral effects of cocaine in rats, emphasizing its role as a promising therapeutic intervention in cocaine abuse.

Indexed as

AdenoviridaeAnimalsAnxietyCarboxylic Ester HydrolasesCocaineCocaine-Related DisordersDietDose-Response Relationship, DrugGenetic TherapyGenetic VectorsMaleMotor ActivityRatsRats, WistarRewardSelf AdministrationCarboxylic Ester HydrolasesCocainecocaine esterase

Identifiers

PMID26968195
PMCPMC4851322
OpenAlexW2296082288

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.