ArticleJournal of visualized experiments : JoVE2016
An Improved Method for Rapid Intubation of the Trachea in Mice.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Transduction Mechanisms for Cold Temperature in Mouse Trigeminal and Vagal Ganglion Neurons Innervating Different Peripheral Organs.Acta physiologica (Oxford, England) · 2025Article
- Surgical Implantation of a Telemetry-Based Pressure Sensor in the Internal Jugular Vein to Monitor Respiration Wirelessly.Bio-protocol · 2025Article
- Article
- Pulmonary lysyl oxidase expression and its role in seeding Lewis lung carcinoma cells.Clinical & experimental metastasis · 2024Article
- Highly efficacious and safe neutralizing DNA aptamer of SARS-CoV-2 as an emerging therapy for COVID-19 disease.Virology journal · 2022Article
- Anesthesia and analgesia for common research models of adult mice.Laboratory animal research · 2022Review
- Lung targeted liposomes for treating ARDS.Journal of controlled release : official journal of the Controlled Release Society · 2022Article
- A Minimally Invasive Method for Intratracheal Instillation of Drugs in Neonatal Rodents to Treat Lung Disease.Journal of visualized experiments : JoVE · 2021Article
- Experimental data of labeling the heart and cardiac cultures with a retrograde tracerData in brief · 2021Article
- Hyperbaric Oxygen Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Mice.Frontiers in molecular biosciences · 2021Article
- Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice.Pharmaceutics · 2020Article
- Determination of reliable lung function parameters in intubated mice.Respiratory research · 2019Article
- Murine Surgical Model of Topical Elastase Induced Descending Thoracic Aortic Aneurysm.Journal of visualized experiments : JoVE · 2019Article
- Optimal tube length of orotracheal intubation for mice.Laboratory animals · 2019Article
- How to monitor breathing in laboratory rodents: a review of the current methods.Journal of neurophysiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity is a commonly used model to study both acute lung injury and fibrosis, and multiple methods have been developed for administering bleomycin (and other toxic agents) into the lungs. However, many of these approaches, such as transtracheal instillation, have inherent drawbacks, including the need for strong anesthetics and survival surgery. This paper reports a quick, reproducible method of intratracheal intubation that involves mild inhaled anesthesia, visualization of the trachea, and the use of a surrogate spirometer to confirm exposure. As a proof of concept, 8-12 week old C57BL/6 mice were administered either 2.0 U/kg of bleomycin or an equivalent volume of PBS, and both damage and fibrotic endpoints were measured post-exposure. This procedure allows researchers to treat a large cohort of mice in a relatively short period with little expense and minimal post-procedure care.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.