ArticleJournal of the American Chemical Society2016
Fluorescence Monitoring of the Oxidative Repair of DNA Alkylation Damage by ALKBH3, a Prostate Cancer Marker.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Correlation between RNA N6-methyladenosine and ferroptosis in cancer: current status and prospects.Frontiers in cell and developmental biology · 2024Review
- Chemical Tools for the Study of DNA Repair.Accounts of chemical research · 2022Article
- Physio-pathological effects of m6A modification and its potential contribution to melanoma.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Review
- Bone marrow mesenchymal stem cells promote prostate cancer cell stemness via cell-cell contact to activate the Jagged1/Notch1 pathway.Cell & bioscience · 2021Article
- Multi-substrate selectivity based on key loops and non-homologous domains: new insight into ALKBH family.Cellular and molecular life sciences : CMLS · 2021Review
- NInternational journal of biological sciences · 2021Review
- Review
- Designer Fluorescent Adenines Enable Real-Time Monitoring of MUTYH Activity.ACS central science · 2020Article
- Fluorescence imaging of drug target proteins using chemical probes.Journal of pharmaceutical analysis · 2020Review
- The role of NMolecular cancer · 2020Review
- An Excimer Clamp for Measuring Damaged-Base Excision by the DNA Repair Enzyme NTH1.Angewandte Chemie (International ed. in English) · 2020Article
- Probing of Nucleic Acid Structures, Dynamics, and Interactions With Environment-Sensitive Fluorescent Labels.Frontiers in chemistry · 2020Review
- Ionizing Radiation and Complex DNA Damage: From Prediction to Detection Challenges and Biological Significance.Cancers · 2019Review
- Fluorescence Probes for ALKBH2 Allow the Measurement of DNA Alkylation Repair and Drug Resistance Responses.Angewandte Chemie (International ed. in English) · 2018Article
- Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging.ACS chemical biology · 2018Review
- Fluorescent Probes of DNA Repair.ACS chemical biology · 2018Review
- Potent and Selective Inhibitors of 8-Oxoguanine DNA Glycosylase.Journal of the American Chemical Society · 2018Article
- Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides.Molecules (Basel, Switzerland) · 2017Review
- Fluorescent nucleobases as tools for studying DNA and RNA.Nature chemistry · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The 2-oxoglutarate-dependent iron enzyme ALKBH3 is an antitumor target and a potential diagnostic marker for several tumor types, including prostate cancer. However, there is at present no simple way to measure this enzyme's activity. Here we describe a fluorogenic probe design (MAQ) that is directly responsive to ALKBH3 repair activity. It makes use of the fluorescence-quenching properties of 1-methyladenine; removal of the alkyl group results in a >10-fold light-up signal. The probe is specific for ALKBH3 over its related homologue ALKBH2 and can be used to identify and measure the effectiveness of enzyme inhibitors. Measurements of the enzyme substrate parameters show that MAQ displays Km and kcat values essentially the same as those of the native substrate. Finally, we show that the probe functions efficiently in cells, allowing imaging and quantitation of ALKBH3 activity by microscopy and flow cytometry. We expect that MAQ probes will be broadly useful in the study of the basic biology of ALKBH3 and in clinical cancer applications as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.