ArticleJournal of visualized experiments : JoVE2016
Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- A novel stress response pathway mediates biofilm architecture in Pseudomonas aeruginosa.PLoS pathogens · 2026Article
- SMC ensures efficient chromosome replication and oriC positioning during Streptomyces spore germination.Scientific reports · 2026Article
- A histidine pseudokinase modulates polar growth and cell shape in Streptomyces venezuelae.Communications biology · 2026Article
- Imaging the Entire Sexual Life Cycle of the Budding YeastBio-protocol · 2025Article
- SMC modulates ParB engagement in segregation complexes in streptomyces.Nature communications · 2025Article
- Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle.Molecular biology of the cell · 2025Article
- Deletion ofMicrobiology (Reading, England) · 2025Article
- A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division.BMC microbiology · 2024Article
- The Best of Both Worlds-Streptomyces coelicolor and Streptomyces venezuelae as Model Species for Studying Antibiotic Production and Bacterial Multicellular Development.Journal of bacteriology · 2023Review
- ActinoBase: tools and protocols for researchers working onMicrobial genomics · 2022Article
- Hyphal compartmentalization and sporulation in Streptomyces require the conserved cell division protein SepX.Nature communications · 2022Article
- Streptomyces venezuelae NRRL B-65442: genome sequence of a model strain used to study morphological differentiation in filamentous actinobacteria.Journal of industrial microbiology & biotechnology · 2021Article
- AdpA Positively Regulates Morphological Differentiation and Chloramphenicol Biosynthesis in Streptomyces venezuelae.Microbiology spectrum · 2021Article
- Heterologous expression of 8-demethyl-tetracenomycin (8-dmtc) affected Streptomyces coelicolor life cycle.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development.Nature communications · 2021Article
- Article
- Article
- Influence of core divisome proteins on cell division inMicrobiology (Reading, England) · 2021Article
- Compaction and control-the role of chromosome-organizing proteins in Streptomyces.FEMS microbiology reviews · 2020Review
- c-di-GMP Arms an Anti-σ to Control Progression of Multicellular Differentiation in Streptomyces.Molecular cell · 2020Article
Corrections and comments
- Erratum issuedErratum: Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device.2016 · [Corrected from Buttner, Mark] [Corrected to Buttner, Mark J.]
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Live-cell imaging of biological processes at the single cell level has been instrumental to our current understanding of the subcellular organization of bacterial cells. However, the application of time-lapse microscopy to study the cell biological processes underpinning development in the sporulating filamentous bacteria Streptomyces has been hampered by technical difficulties. Here we present a protocol to overcome these limitations by growing the new model species, Streptomyces venezuelae, in a commercially available microfluidic device which is connected to an inverted fluorescence widefield microscope. Unlike the classical model species, Streptomyces coelicolor, S. venezuelae sporulates in liquid, allowing the application of microfluidic growth chambers to cultivate and microscopically monitor the cellular development and differentiation of S. venezuelae over long time periods. In addition to monitoring morphological changes, the spatio-temporal distribution of fluorescently labeled target proteins can also be visualized by time-lapse microscopy. Moreover, the microfluidic platform offers the experimental flexibility to exchange the culture medium, which is used in the detailed protocol to stimulate sporulation of S. venezuelae in the microfluidic chamber. Images of the entire S. venezuelae life cycle are acquired at specific intervals and processed in the open-source software Fiji to produce movies of the recorded time-series.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.