ArticleACS nano2016
Molecular Occupancy of Nanodot Arrays.
Article in ACS nano, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- G Protein-Coupled Estrogen Receptor Regulates Mesenchymal Stem Cell Mechanotransduction and Differentiation.Cellular and molecular bioengineering · 2025Article
- Plasmonic DNA-Barcoded Virion Nano-Oscillators for Multiplexed Quantification of Small-Molecule Binding Kinetics to Membrane Proteins.Angewandte Chemie (International ed. in English) · 2025Article
- Frontiers in mechanobiology and mechanomedicine.Med-X · 2025Article
- TiOAdvanced materials (Deerfield Beach, Fla.) · 2024Article
- Intrinsic self-organization of integrin nanoclusters within focal adhesions is required for cellular mechanotransduction.bioRxiv : the preprint server for biology · 2023Article
- Fabrication of Nanoscale Arrays to Study the Effect of Ligand Arrangement on Inhibitory Signaling in NK Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ligand functionalization of titanium nanopattern enables the analysis of cell-ligand interactions by super-resolution microscopy.Nature protocols · 2022Review
- Molecular-scale spatio-chemical control of the activating-inhibitory signal integration in NK cells.Science advances · 2021Article
- The CAR T-Cell Mechanoimmunology at a Glance.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Integrin nanoclusters can bridge thin matrix fibres to form cell-matrix adhesions.Nature materials · 2019Article
- Advanced Materials and Devices for the Regulation and Study of NK Cells.International journal of molecular sciences · 2019Review
- Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors.Analytical chemistry · 2018Article
- Full control of ligand positioning reveals spatial thresholds for T cell receptor triggering.Nature nanotechnology · 2018Article
- Activation of Human Natural Killer Cells by Graphene Oxide-Templated Antibody Nanoclusters.Nano letters · 2018Article
- Fabrication of Nanoscale Pits with High Throughput on Polymer Thin Film Using AFM Tip-Based Dynamic Plowing Lithography.Nanoscale research letters · 2017Article
- Improved Glass Surface Passivation for Single-Molecule Nanoarrays.Langmuir : the ACS journal of surfaces and colloids · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Single-molecule nanodot arrays, in which a biomolecule of choice (protein, nucleic acid, etc.) is bound to a metallic nanoparticle on a solid substrate, are becoming an increasingly important tool in the study of biomolecular and cellular interactions. We have developed an on-chip measurement protocol to monitor and control the molecular occupancy of nanodots. Arrays of widely spaced nanodots and nanodot clusters were fabricated on glass surfaces by nanolithography and functionalized with fluorescently labeled proteins. The molecular occupancy was determined by monitoring individual fluorophore bleaching events, while accounting for fluorescence quenching effects. We found that the occupancy can be interpreted as a packing problem, and depends on nanodot size and binding ligand concentration, where the latter is easily adjusted to compensate the flexibility of dimension control in nanofabrication. The results are scalable with nanodot cluster size, extending to large area close packed arrays. As an example, the nanoarray platform was used to probe the geometric requirement of T-cell activation at the single-molecule level.
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Registered trials
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