ArticlePlant physiology2016
A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 53 citations in OpenAlex.
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- Identifying and Visualizing Novel Small Open Reading Frames (sORFs) Via Ribo-Seq, Transcriptome Assembly, and ggRibo.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Multiomics analysis revealed the temporally common and specific molecular changes in Arabidopsis thaliana (L.) under salt stress.BMC genomics · 2025Article
- Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors.Nucleic acids research · 2025Article
- Discovery of diverse chimeric peptides in a eukaryotic proteome sets the stage for experimental validation of the mosaic translation hypothesis.Computational and structural biotechnology journal · 2025Article
- ChiMSource improves the accuracy of studies on novel amino acid sequences by predicting alternative sources of mass spectrometry-derived peptides.Computational and structural biotechnology journal · 2025Article
- Ribosome binding of phasiRNA precursors accelerates the 24-nt phasiRNA burst in meiotic maize anthers.The Plant cell · 2024Article
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- What, where, and how: Regulation of translation and the translational landscape in plants.The Plant cell · 2024Review
- Improved super-resolution ribosome profiling reveals prevalent translation of upstream ORFs and small ORFs in Arabidopsis.The Plant cell · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in Arabidopsis.Nucleic acids research · 2022Article
- Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- Evolution of PHAS loci in the young spike of Allohexaploid wheat.BMC genomics · 2020Article
- Gene Regulation Mediated by microRNA-Triggered Secondary Small RNAs in Plants.Plants (Basel, Switzerland) · 2019Review
- Biogenesis and regulatory hierarchy of phased small interfering RNAs in plants.Plant biotechnology journal · 2018Review
- The 'how' and 'where' of plant microRNAs.The New phytologist · 2017Review
- Global analysis of ribosome-associated noncoding RNAs unveils new modes of translational regulation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of 5'-capped and 3'-polyadenylated primary TAS transcripts (pri-TASs). Although pri-TASs are not thought to encode functional proteins, they contain multiple short open reading frames (ORFs). For example, the primary TAS2 transcript (pri-TAS2) contains 11 short ORFs, and the third ORF from the 5' terminus (ORF3) encompasses the miR173 target site. Here, we show that nonsense mutations in ORF3 of pri-TAS2 upstream of the miR173 recognition site suppress tasiRNA accumulation and that ORF3 is translated in vitro. Glycerol gradient centrifugation analysis of Arabidopsis (Arabidopsis thaliana) plant extracts revealed that pri-TAS2 and its miR173-cleaved 5' and 3' fragments are fractionated together in the polysome fractions. These and previous results suggest that the 3' fragment of pri-TAS2, which is a source of tasiRNAs, forms a huge complex containing SGS3, miR173-programmed AGO1 RNA-induced silencing complex, the 5' fragment, and ribosomes. This complex overaccumulated, moderately accumulated, and did not accumulate in rdr6, sde5, and sgs3 mutants, respectively. The sgs3 sde5 and rdr6 sde5 double mutants showed phenotypes similar to those of sgs3 and sde5 single mutants, respectively, with regard to the TAS2-related RNA accumulation, suggesting that the complex is formed in an SGS3-dependent manner, somehow modified and stabilized by SDE5, and becomes competent for RDR6 action. Ribosomes in this complex likely play an important role in this process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.