ArticleScience (New York, N.Y.)2016
Measurement of gene regulation in individual cells reveals rapid switching between promoter states.
Article in Science (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Cell Growth and Division Shape mRNA-Protein Correlations.bioRxiv : the preprint server for biology · 2026Article
- Efficient approximations of transcriptional bursting effects on the dynamics of a gene regulatory network.Journal of the Royal Society, Interface · 2025Article
- Sequestration of gene products by decoys enhances precision in the timing of intracellular events.Scientific reports · 2024Article
- Article
- Solving stochastic gene-expression models using queueing theory: A tutorial review.Biophysical journal · 2024Review
- Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing.STAR protocols · 2024Article
- Transcriptional bursting dynamics in gene expression.Frontiers in genetics · 2024Review
- Article
- Poisson representation: a bridge between discrete and continuous models of stochastic gene regulatory networks.Journal of the Royal Society, Interface · 2023Article
- Dominance vs epistasis: the biophysical origins and plasticity of genetic interactions within and between alleles.Nature communications · 2023Article
- Inferring transcriptional bursting kinetics from single-cell snapshot data using a generalized telegraph model.Royal Society open science · 2023Article
- BdLT-Seq as a barcode decay-based method to unravel lineage-linked transcriptome plasticity.Nature communications · 2023Article
- Gene expression model inference from snapshot RNA data using Bayesian non-parametrics.Nature computational science · 2023Article
- Article
- Analysis and design of single-cell experiments to harvest fluctuation information while rejecting measurement noise.Frontiers in cell and developmental biology · 2023Article
- Article
- Molecular Origins of Transcriptional Heterogeneity in Diazotrophic Klebsiella oxytoca.mSystems · 2022Article
- Research progress of live-cell RNA imaging techniques.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Tunable transcription factor library for robust quantification of regulatory properties in Escherichia coli.Molecular systems biology · 2022Article
- Quantifying biochemical reaction rates from static population variability within incompletely observed complex networks.PLoS computational biology · 2022Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
In vivo mapping of transcription-factor binding to the transcriptional output of the regulated gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies, and cell-to-cell variability. We demonstrate how to overcome these obstacles in the lysogeny maintenance promoter of bacteriophage lambda, P(RM). We simultaneously measured the concentration of the lambda repressor CI and the number of messenger RNAs (mRNAs) from P(RM) in individual Escherichia coli cells, and used a theoretical model to identify the stochastic activity corresponding to different CI binding configurations. We found that switching between promoter configurations is faster than mRNA lifetime and that individual gene copies within the same cell act independently. The simultaneous quantification of transcription factor and promoter activity, followed by stochastic theoretical analysis, provides a tool that can be applied to other genetic circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.