Evidence map›Paper›PMID 26965162›Full record

ArticleJournal of the American Society for Mass Spectrometry2016

Differential Mobility Spectrometry-Hydrogen Deuterium Exchange (DMS-HDX) as a Probe of Protein Conformation in Solution.

Shaolong Zhu, J Larry Campbell, Igor Chernushevich, J C Yves Le Blanc, Derek J Wilson

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 21 citations in OpenAlex.

  1. Review
  2. Article
  3. Structural evaluations of tau protein conformation: methodologies and approaches.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2017
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shaolong ZhuChemistry Department, York University, Toronto, ON, M3J 1P3, Canada.
J Larry CampbellSCIEX, 71 Four Valley Drive, Concord, ON, L4K 4V8, Canada.
Igor ChernushevichSCIEX, 71 Four Valley Drive, Concord, ON, L4K 4V8, Canada.
J C Yves Le BlancSCIEX, 71 Four Valley Drive, Concord, ON, L4K 4V8, Canada.
Derek J WilsonChemistry Department, York University, Toronto, ON, M3J 1P3, Canada. dkwilson@yorku.ca.
York University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Differential mobility spectrometry (DMS) is an ion mobility technique that has been adopted chiefly as a pre-filter for small- to medium-sized analytes (<1 000 Da). With the exception of a handful of studies that employ an analogue of DMS-field asymmetric waveform ion mobility spectroscopy (FAIMS)-the application of DMS to intact biomacromolecules remains largely unexplored. In this work, we employ DMS combined with gas-phase hydrogen deuterium exchange (DMS-HDX) to probe the gas-phase conformations generated from proteins that were initially folded, partially-folded, and unfolded in solution. Our findings indicate that proteins with distinct structural features in solution exhibit unique deuterium uptake profiles as function of their optimal transmission through the DMS. Ultimately we propose that DMS-HDX can, if properly implemented, provide rapid measurements of liquid-phase protein structural stability that could be of use in biopharmaceuticals development. Graphical Abstract ᅟ.

Indexed as

Deuterium Exchange MeasurementProtein ConformationDeuteriumHydrogenSpectrum AnalysisDeuteriumHydrogenCytochrome cDifferential mobility spectrometryDMSGas phase hydrogen deuterium exchangeHDXHydrogen deuterium exchangeIon mobilityMass spectrometryMyoglobinProtein structureTauTau protein

Identifiers

PMID26965162
OpenAlexW2294987518

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.