ArticleMethods in molecular biology (Clifton, N.J.)2016
Combined Optogenetic and Chemogenetic Control of Neurons.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Subcutaneous Neuromodulation Activates Central Pain-Suppression Pathways for Analgesia.CNS neuroscience & therapeutics · 2026Article
- Strength of activation and temporal dynamics of bioluminescent-optogenetics in response to systemic injections of the luciferin.NeuroImage · 2024Article
- Imaging bioluminescence by detecting localized haemodynamic contrast from photosensitized vasculature.Nature biomedical engineering · 2024Article
- Unlocking the potential of adeno-associated virus in neuroscience: a brief review.Molecular biology reports · 2024Review
- BioLuminescent OptoGenetics in the choroid plexus: integrated opto- and chemogenetic controlNeurophotonics · 2024Article
- Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes.International journal of molecular sciences · 2023Review
- Experimental approaches for manipulating choroid plexus epithelial cells.Fluids and barriers of the CNS · 2022Review
- Engineering Brain Organoids: Toward Mature Neural Circuitry with an Intact Cytoarchitecture.International journal of stem cells · 2022Review
- The emergence of molecular systems neuroscience.Molecular brain · 2022Review
- Autonomic dysfunction in epilepsy mouse models with implications for SUDEP research.Frontiers in neurology · 2022Review
- Bioluminescent Optogenetics: A Novel Experimental Therapy to Promote Axon Regeneration after Peripheral Nerve Injury.International journal of molecular sciences · 2021Article
- New Insights of SF1 Neurons in Hypothalamic Regulation of Obesity and Diabetes.International journal of molecular sciences · 2021Review
- Nanoscale programming of cellular and physiological phenotypes: inorganic meets organic programming.NPJ systems biology and applications · 2021Review
- Electrophysiological Phenotype Characterization of Human iPSC-Derived Neuronal Cell Lines by Means of High-Density Microelectrode Arrays.Advanced biology · 2021Article
- Restoring Function After Severe Spinal Cord Injury Through BioLuminescent-OptoGenetics.Frontiers in neurology · 2021Article
- Motoneuron activity is required for enhancements in functional recovery after peripheral nerve injury in exercised female mice.Journal of neuroscience research · 2020Article
- The BioLuminescent-OptoGenetic in vivo response to coelenterazine is proportional, sensitive, and specific in neocortex.Journal of neuroscience research · 2020Article
- Defining parameters of specificity for bioluminescent optogenetic activation of neurons using in vitro multi electrode arrays (MEA).Journal of neuroscience research · 2020Article
- Bioluminescence-driven optogenetic activation of transplanted neural precursor cells improves motor deficits in a Parkinson's disease mouse model.Journal of neuroscience research · 2020Article
- Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems.ACS synthetic biology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Optogenetics provides an array of elements for specific biophysical control, while designer chemogenetic receptors provide a minimally invasive method to control circuits in vivo by peripheral injection. We developed a strategy for selective regulation of activity in specific cells that integrates opto- and chemogenetic approaches, and thus allows manipulation of neuronal activity over a range of spatial and temporal scales in the same experimental animal. Light-sensing molecules (opsins) are activated by biologically produced light through luciferases upon peripheral injection of a small molecule substrate. Such luminescent opsins, luminopsins, allow conventional fiber optic use of optogenetic sensors, while at the same time providing chemogenetic access to the same sensors. We describe applications of this approach in cultured neurons in vitro, in brain slices ex vivo, and in awake and anesthetized animals in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.