ArticleNature communications2016
Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- ZDHHC5: a pivotal palmitoyltransferase orchestrating signaling networks - unraveling mechanisms and therapeutic horizons.Biomarker research · 2026Review
- Biosynthesis of the fragrance compound cinnamyl isobutyrate in Escherichia coli.Biotechnology letters · 2026Article
- Aminolipids in bacterial membranes and the natural environment.The ISME journal · 2024Review
- Acylation of glycerolipids in mycobacteria.Nature communications · 2023Article
- PatA Regulates Isoniazid Resistance by Mediating Mycolic Acid Synthesis and Controls Biofilm Formation by Affecting Lipid Synthesis in Mycobacteria.Microbiology spectrum · 2023Article
- Molecular ruler mechanism and interfacial catalysis of the integral membrane acyltransferase PatA.Science advances · 2021Article
- Mutagenesis and homology modeling reveal a predicted pocket of lysophosphatidylcholine acyltransferase 2 to catch Acyl-CoA.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- The Phosphatidyl-Journal of bacteriology · 2021Article
- The thick waxy coat of mycobacteria, a protective layer against antibiotics and the host's immune system.The Biochemical journal · 2020Review
- The Two-Component Locus MSMEG_0244/0246 Together With MSMEG_0243 Affects Biofilm Assembly inFrontiers in microbiology · 2020Article
- Transcriptional signatures of Mycobacterium tuberculosis in mouse model of intraocular tuberculosis.Pathogens and disease · 2019Article
- Rickettsia Lipid A Biosynthesis Utilizes the Late Acyltransferase LpxJ for Secondary Fatty Acid Addition.Journal of bacteriology · 2018Article
- Structural basis for lipid binding and mechanism of theThe Journal of biological chemistry · 2018Article
- Article
- How lipid droplets "TAG" along: Glycerolipid synthetic enzymes and lipid storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- A two-helix motif positions the lysophosphatidic acid acyltransferase active site for catalysis within the membrane bilayer.Nature structural & molecular biology · 2017Article
- Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 4 countries.
Funding
Abstract
The biosynthesis of phospholipids and glycolipids are critical pathways for virtually all cell membranes. PatA is an essential membrane associated acyltransferase involved in the biosynthesis of mycobacterial phosphatidyl-myo-inositol mannosides (PIMs). The enzyme transfers a palmitoyl moiety from palmitoyl-CoA to the 6-position of the mannose ring linked to 2-position of inositol in PIM1/PIM2. We report here the crystal structures of PatA from Mycobacterium smegmatis in the presence of its naturally occurring acyl donor palmitate and a nonhydrolyzable palmitoyl-CoA analog. The structures reveal an α/β architecture, with the acyl chain deeply buried into a hydrophobic pocket that runs perpendicular to a long groove where the active site is located. Enzyme catalysis is mediated by an unprecedented charge relay system, which markedly diverges from the canonical HX4D motif. Our studies establish the mechanistic basis of substrate/membrane recognition and catalysis for an important family of acyltransferases, providing exciting possibilities for inhibitor design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.