ArticleFree radical biology & medicine2016
Reinterpreting the best biomarker of oxidative stress: The 8-iso-prostaglandin F2α/prostaglandin F2α ratio shows complex origins of lipid peroxidation biomarkers in animal models.
Article in Free radical biology & medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 2 of them syntheses that pooled it.
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Who cites it
47 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- Strategies to decrease oxidative stress biomarker levels in human medical conditions: A meta-analysis on 8-iso-prostaglandin FRedox biology · 2018Pooled it
- Classifying oxidative stress by FRedox biology · 2017Pooled it
- Trial
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- Circulating GPER-1, 8-Iso-Prostaglandin FJournal of clinical medicine · 2026Article
- Early Epigenetic and Metabolic Responses to the Adipocyte Secretome Reveal Stress-Adaptive States in Triple-Negative Breast Cancer.bioRxiv : the preprint server for biology · 2026Article
- Analysis of Reactive Oxygen Species-Induced Cellular Damage in Cervical Cancer.Current protocols · 2026Article
- Overview of the effect of dietary supplementation with plant sterols on low-grade inflammation, endothelial dysfunction and oxidative stress in patients with hypercholesterolemia.Lipids in health and disease · 2026Review
- Next-Generation Antioxidants in Cardiovascular Disease: Mechanistic Insights and Emerging Therapeutic Strategies.Antioxidants (Basel, Switzerland) · 2026Review
- Isoprostanes as biomarkers of oxidative stress across human diseases and skin pathologies.Frontiers in molecular biosciences · 2026Review
- Phthalates exposure and serum uric acid level in patients with Crohn's disease: A cross-sectional study.PloS one · 2026Article
- Optimal timing to assess exercise-induced oxidative stress: A systematic review and meta-analysis.Experimental physiology · 2025Review
- Assessment of per- and polyfluoroalkyl substances (PFAS) exposure and associations with oxidative stress biomarkers among pregnant women from the PROTECT cohort.The Science of the total environment · 2025Article
- Rapid Lipid Mediator Profiling by Convergence Chromatography-MS/MS.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Blood cadmium concentration and pulmonary function injury: potential mediating role of oxidative stress in chronic obstructive pulmonary disease patients.BMC pulmonary medicine · 2024Article
- Role of Oxidative Stress in the Occurrence and Development of Cognitive Dysfunction in Patients with Obstructive Sleep Apnea Syndrome.Molecular neurobiology · 2024Review
- Human In Vitro Oxidized Low-Density Lipoprotein (oxLDL) Increases Urinary Albumin Excretion in Rats.International journal of molecular sciences · 2024Article
- Health position paper and redox perspectives - Disease burden by transportation noise.Redox biology · 2024Review
- Effect of SophorOxDiabetes, metabolic syndrome and obesity : targets and therapy · 2024Article
- Artificial intelligence-based clustering and characterization of Parkinson's disease trajectories.Scientific reports · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Oxidative stress is elevated in numerous environmental exposures and diseases. Millions of dollars have been spent to try to ameliorate this damaging process using anti-oxidant therapies. Currently, the best accepted biomarker of oxidative stress is the lipid oxidation product 8-iso-prostaglandin F2α (8-iso-PGF2α), which has been measured in over a thousand human and animal studies. 8-iso-PGF2α generation has been exclusively attributed to nonenzymatic chemical lipid peroxidation (CLP). However, 8-iso-PGF2α can also be produced enzymatically by prostaglandin-endoperoxide synthases (PGHS) in vivo. When failing to account for PGHS-dependent generation, 8-iso-PGF2α cannot be interpreted as a selective biomarker of oxidative stress. We investigated the formation of 8-iso-PGF2α in rats exposed to carbon tetrachloride (CCl4) or lipopolysaccharide (LPS) using the 8-iso-PGF2α/PGF2α ratio to quantitatively determine the source(s) of 8-iso-PGF2α. Upon exposure to a 120mg/kg dose of CCl4, the contribution of CLP accounted for only 55.6±19.4% of measured 8-iso-PGF2α, whereas in the 1200mg/kg dose, CLP was the predominant source of 8-iso-PGF2α (86.6±8.0% of total). In contrast to CCl4, exposure to 0.5mg/kg LPS was characterized by a significant increase in both the contribution of PGHS (59.5±7.0) and CLP (40.5±14.0%). In conclusion, significant generation of 8-iso-PGF2α occurs through enzymatic as well as chemical lipid peroxidation. The distribution of the contribution is dependent on the exposure agent as well as the dose. The 8-iso-PGF2α/PGF2α ratio accurately determines the source of 8-iso-PGF2α and provides an absolute measure of oxidative stress in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.