ArticlePloS one2016
Evolution of E. coli on [U-13C]Glucose Reveals a Negligible Isotopic Influence on Metabolism and Physiology.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Systematic comparison of local approaches for isotopically nonstationary metabolic flux analysis.Frontiers in plant science · 2023Pooled it
- Counterintuitive method improves yields of isotopically labelled proteins expressed in flask-cultured Escherichia coli.Journal of biomolecular NMR · 2025Article
- Predicting the Rate Structure of an Evolved Metabolic Network.Metabolites · 2025Article
- Challenges and recent advances in quantitative mass spectrometry-based metabolomics.Analytical science advances · 2024Review
- Mid-Infrared Photothermal-Fluorescence In Situ Hybridization for Functional Analysis and Genetic Identification of Single Cells.Analytical chemistry · 2023Article
- Article
- Rapid Adaptation Often Occurs through Mutations to the Most Highly Conserved Positions of the RNA Polymerase Core Enzyme.Genome biology and evolution · 2022Article
- Synergistic Effect of β-Lapachone and Aminooxyacetic Acid on Central Metabolism in Breast Cancer.Nutrients · 2022Article
- Global pleiotropic effects in adaptively evolvedOpen biology · 2022Article
- Autotrophic and mixotrophic metabolism of an anammox bacterium revealed by in vivoThe ISME journal · 2021Article
- Bacterial fitness landscapes stratify based on proteome allocation associated with discrete aero-types.PLoS computational biology · 2021Article
- Developing rapid growingMetabolic engineering communications · 2020Article
- Causal mutations from adaptive laboratory evolution are outlined by multiple scales of genome annotations and condition-specificity.BMC genomics · 2020Article
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms.Journal of microbiology and biotechnology · 2020Review
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Pseudogene repair driven by selection pressure applied in experimental evolution.Nature microbiology · 2019Article
- ALEdb 1.0: a database of mutations from adaptive laboratory evolution experimentation.Nucleic acids research · 2019Article
- Continuous Adaptive Evolution of a Fast-GrowingFrontiers in microbiology · 2019Article
- How adaptive evolution reshapes metabolism to improve fitness: recent advances and future outlook.Current opinion in chemical engineering · 2018Article
- Experimental evolution of diverse Escherichia coli metabolic mutants identifies genetic loci for convergent adaptation of growth rate.PLoS genetics · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
13C-Metabolic flux analysis (13C-MFA) traditionally assumes that kinetic isotope effects from isotopically labeled compounds do not appreciably alter cellular growth or metabolism, despite indications that some biochemical reactions can be non-negligibly impacted. Here, populations of Escherichia coli were adaptively evolved for ~1000 generations on uniformly labeled 13C-glucose, a commonly used isotope for 13C-MFA. Phenotypic characterization of these evolved strains revealed ~40% increases in growth rate, with no significant difference in fitness when grown on either labeled (13C) or unlabeled (12C) glucose. The evolved strains displayed decreased biomass yields, increased glucose and oxygen uptake, and increased acetate production, mimicking what is observed after adaptive evolution on unlabeled glucose. Furthermore, full genome re-sequencing revealed that the key genetic changes underlying these phenotypic alterations were essentially the same as those acquired during adaptive evolution on unlabeled glucose. Additionally, glucose competition experiments demonstrated that the wild-type exhibits no isotopic preference for unlabeled glucose, and the evolved strains have no preference for labeled glucose. Overall, the results of this study indicate that there are no significant differences between 12C and 13C-glucose as a carbon source for E. coli growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.