ArticleNature protocols2016
Microfluidic differential immunocapture biochip for specific leukocyte counting.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 50 citations in OpenAlex.
- Multiplexed electrical detection of whole viruses from plasma in a microfluidic platform.The Analyst · 2024Article
- Review
- Thin flexible lab-on-a-film for impedimetric sensing in biomedical applications.Scientific reports · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Fully Automated, Sample-to-Answer Leukocyte Functional Assessment Platform for Continuous Sepsis Monitoring via Microliters of Blood.ACS sensors · 2021Article
- Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel.Scientific reports · 2021Article
- Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection.Viruses · 2020Review
- Point of care technologies for sepsis diagnosis and treatment.Lab on a chip · 2019Review
- Microfluidic flow cytometry: The role of microfabrication methodologies, performance and functional specification.Technology · 2018Article
- Monitoring Neutropenia for Cancer Patients at the Point of Care.Small methods · 2017Article
- A point-of-care microfluidic biochip for quantification of CD64 expression from whole blood for sepsis stratification.Nature communications · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Enumerating specific cell types from whole blood can be very useful for research and diagnostic purposes-e.g., for counting of CD4 and CD8 T cells in HIV/AIDS diagnostics. We have developed a biosensor based on a differential immunocapture technology to enumerate specific cells in 30 min using 10 μl of blood. This paper provides a comprehensive stepwise protocol to replicate our biosensor for CD4 and CD8 cell counts. The biochip can also be adapted to enumerate other specific cell types such as somatic cells or cells from tissue or liquid biopsies. Capture of other specific cells requires immobilization of their corresponding antibodies within the capture chamber. Therefore, this protocol is useful for research into areas surrounding immunocapture-based biosensor development. The biosensor production requires 24 h, a one-time cell capture optimization takes 6-9 h, and the final cell counting experiment in a laboratory environment requires 30 min to complete.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.