ArticlePloS one2016
Genetic and Functional Analysis of the Biosynthesis of a Non-Ribosomal Peptide Siderophore in Burkholderia xenovorans LB400.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 24 citations in OpenAlex.
- Siderophore-metal complexes in Paraburkholderia phymatum: structure elucidation of phymabactin.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- Enhancing multi-season wheat yield through plant growth-promoting rhizobacteria using consortium and individual isolate applications.Folia microbiologica · 2025Article
- Versatile sugar and valerate metabolic pathways in Paraburkholderia xenovorans LB400 enable tailored poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production.Applied microbiology and biotechnology · 2025Article
- Surfing in the storm: how Paraburkholderia xenovorans thrives under stress during biodegradation of toxic aromatic compounds and other stressors.FEMS microbiology reviews · 2025Review
- Microbial Siderophores: A New Insight on Healthcare Applications.BME frontiers · 2025Review
- Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile BacteriumPlants (Basel, Switzerland) · 2024Article
- Isolation of a novelFrontiers in microbiology · 2024Article
- Extracytoplasmic Function Sigma Factors Governing Production of the Primary Siderophores in PathogenicFrontiers in microbiology · 2022Review
- Genome-Wide Metabolic Reconstruction of the Synthesis of Polyhydroxyalkanoates from Sugars and Fatty Acids byMicroorganisms · 2021Review
- Comparative genome analyses ofMicrobial genomics · 2020Article
- Delineation of a Subgroup of the Genus Paraburkholderia, Including P. terrae DSM 17804T, P. hospita DSM 17164T, and Four Soil-Isolated Fungiphiles, Reveals Remarkable Genomic and Ecological Features-Proposal for the Definition of a P. hospita Species Cluster.Genome biology and evolution · 2020Article
- Genomic analysis of siderophore β-hydroxylases reveals divergent stereocontrol and expands the condensation domain family.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Determination of the potency of hexyl-ciprofloxacin molecules that interact with gold nanoparticles in a reversible manner.IET nanobiotechnology · 2019Article
- β-Hydroxyaspartic acid in siderophores: biosynthesis and reactivity.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Iron Acquisition Mechanisms and Their Role in the Virulence ofFrontiers in cellular and infection microbiology · 2017Review
- Article
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6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
B. xenovorans LB400 is a model bacterium for the study of the metabolism of aromatic compounds. The aim of this study was the genomic and functional characterization of a non-ribosomal peptide synthetase containing gene cluster that encodes a siderophore in B. xenovorans LB400. The mba gene cluster from strain LB400 encodes proteins involved in the biosynthesis and transport of a hydroxamate-type siderophore. Strain LB400 has a unique mba gene organization, although mba gene clusters have been observed in diverse Burkholderiales. Bioinformatic analysis revealed the presence of promoters in the mba gene cluster that strongly suggest regulation by the ferric uptake regulator protein (Fur) and by the alternative RNA polymerase extracytoplasmic function sigma factor MbaF. Reverse transcriptase PCR analyses showed the expression of iron-regulated transcriptional units mbaFGHIJKL, mbaN, mbaABCE, mbaO, mbaP and mbaD genes under iron limitation. Chrome azurol S (CAS) assay strongly suggests that strain LB400 synthesized a siderophore under iron limitation. Mass spectrometry ESI-MS and MALDI-TOF-MS analyses revealed that the siderophore is a non-ribosomal peptide, and forms an iron complex with a molecular mass of 676 Da. Based on bioinformatic prediction, CAS assay and MS analyses, we propose that the siderophore is L-Nδ-hydroxy-Nδ-formylOrn-D-β-hydroxyAsp-L-Ser-L-Nδ-hydroxy-Nδ-formylOrn-1,4-diaminobutane that is closely related to malleobactin-type siderophores reported in B. thailandensis.
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