ArticlePloS one2016
Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Spaceflight multi-omics reveals vulnerabilities of human germ cell development.Science advances · 2026Article
- Advanced three-dimensional culture technologies in reproductive medicine: A narrative review.International journal of reproductive biomedicine · 2026Review
- Ovarian Reproductive and Endocrine Function Investigated in Microgravity Environment.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The Critical Role of Enhanced OXPHOS and Mitochondrial Hyperpolarization in Simulated Microgravity-Induced Oocyte Maturation Arrest.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Beyond Earth, Beyond Time: Preserving Female Fertility in Space Missions.Journal of clinical medicine · 2025Review
- Autonomous, miniature research station (lab-payload) for the nanosatellite biological mission: LabSat.Scientific reports · 2025Article
- Microgravity and Cellular Biology: Insights into Cellular Responses and Implications for Human Health.International journal of molecular sciences · 2025Review
- Microgravity impairs endocrine signaling and reproductive health of women. A narrative review.Frontiers in physiology · 2025Review
- Beyond Earth's bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space.Reproductive biology and endocrinology : RB&E · 2024Review
- Influence of gut microbiome on metabolic diseases: a new perspective based on microgravity.Journal of diabetes and metabolic disorders · 2024Review
- Article
- Addressing Spaceflight Biology through the Lens of a Histologist-Embryologist.Life (Basel, Switzerland) · 2023Review
- The State of the Organs of the Female Reproductive System after a 5-Day "Dry" Immersion.International journal of molecular sciences · 2023Article
- Simulated microgravity reduces quality of ovarian follicles and oocytes by disrupting communications of follicle cells.NPJ microgravity · 2023Article
- Article
- Single Cell in a Gravity Field.Life (Basel, Switzerland) · 2022Review
- Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells.Current issues in molecular biology · 2021Article
- Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.Science advances · 2021Article
- Mitochondrial Respiration inCurrent issues in molecular biology · 2021Article
- Reproductive hazards of space travel in women and men.Nature reviews. Endocrinology · 2019Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrowing cells in simulated weightlessness condition might be a highly promising new technique to maintain or generate tissue constructs in a scaffold-free manner. There is limited evidence that microgravity condition may affect development of ovarian follicles. The objective of the present study was to investigate the effects of simulated microgravity on the in vitro development of mouse preantral follicles. METHODS AND
resultsOvarian tissue from 14-day-old mice, or preantral follicles mechanically isolated from 14-day-old mouse ovaries were cultured at a simulated microgravity condition generated using a rotating wall vessel apparatus. Follicle survival was assessed quantitatively using H&E staining. Follicle diameter and oocyte diameter were measured under an inverted microscope. Ultrastructure of oocytes was evaluated using transmission electron microscopy. We observed that simulated microgravity compromised follicle survival in vitro, downregulated PCNA and GDF-9 expressions, and caused ultrastructural abnormalities in oocytes.
conclusionThis study showed for the first time that three-dimensional culture condition generated by simulated microgravity is detrimental to the initial stage development of mouse preantral follicles in vitro. The experimental setup provides a model to further investigate the mechanisms involved in the in vitro developmental processes of oocytes/granulosa cells under the microgravity condition.
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