Evidence map›Paper›PMID 26962854›Full record

Trial reportPloS one2016

High-Density Lipoprotein-Associated miR-223 Is Altered after Diet-Induced Weight Loss in Overweight and Obese Males.

Fatiha Tabet, Luisa F Cuesta Torres, Kwok Leung Ong, Sudichhya Shrestha, Sébastien A Choteau, Philip J Barter, Peter Clifton, Kerry-Anne Rye

Open access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.

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  19. Advances in HDL: Much More than Lipid Transporters.International journal of molecular sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Fatiha TabetLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Luisa F Cuesta TorresLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Kwok Leung OngLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Sudichhya ShresthaLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Sébastien A ChoteauLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Philip J BarterLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
Peter CliftonSchool of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, South Australia, Australia.
Kerry-Anne RyeLipid Research Group, School of Medical Sciences, University of New South Wales Australia, Randwick, New South Wales, Australia.
UNSW Sydney · AUUniversity of South Australia · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimsmicroRNAs (miRNAs) are small, endogenous non-coding RNAs that regulate metabolic processes, including obesity. The levels of circulating miRNAs are affected by metabolic changes in obesity, as well as in diet-induced weight loss. Circulating miRNAs are transported by high-density lipoproteins (HDL) but the regulation of HDL-associated miRNAs after diet-induced weight loss has not been studied. We aim to determine if HDL-associated miR-16, miR-17, miR-126, miR-222 and miR-223 levels are altered by diet-induced weight loss in overweight and obese males.

methodsHDL were isolated from 47 subjects following 12 weeks weight loss comparing a high protein diet (HP, 30% of energy) with a normal protein diet (NP, 20% of energy). HDL-associated miRNAs (miR-16, miR-17, miR-126, miR-222 and miR-223) at baseline and after 12 weeks of weight loss were quantified by TaqMan miRNA assays. HDL particle sizes were determined by non-denaturing polyacrylamide gradient gel electrophoresis. Serum concentrations of human HDL constituents were measured immunoturbidometrically or enzymatically.

resultsmiR-16, miR-17, miR-126, miR-222 and miR-223 were present on HDL from overweight and obese subjects at baseline and after 12 weeks of the HP and NP weight loss diets. The HP diet induced a significant decrease in HDL-associated miR-223 levels (p = 0.015), which positively correlated with changes in body weight (r = 0.488, p = 0.032). Changes in miR-223 levels were not associated to changes in HDL composition or size.

conclusionHDL-associated miR-223 levels are significantly decreased after HP diet-induced weight loss in overweight and obese males. This is the first study reporting changes in HDL-associated miRNA levels with diet-induced weight loss.

Indexed as

Weight LossAdultHumansLipoproteins, HDLMaleMicroRNAsMiddle AgedObesityReal-Time Polymerase Chain ReactionLipoproteins, HDLMicroRNAsMIR223, human

Identifiers

PMID26962854
PMCPMC4786204
OpenAlexW2293424103

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.